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Suh, Pann-Ghill
BioSignal Network Lab (BSN)
Research Interests
  • Signal transduction, cancer, metabolism, phospholipase C

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S-PHASE INDUCTION AND TRANSFORMATION OF QUIESCENT NIH-3T3 CELLS BY MICROINJECTION OF PHOSPHOLIPASE-C

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Title
S-PHASE INDUCTION AND TRANSFORMATION OF QUIESCENT NIH-3T3 CELLS BY MICROINJECTION OF PHOSPHOLIPASE-C
Author
Smith, Mark R.Ryu, Sung-HoSuh, Pann-GhillRhee, Sue-GooKung, Hsiang-Fu
Issue Date
1989-05
Publisher
NATL ACAD SCIENCES
Citation
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v.86, no.10, pp.3659 - 3663
Abstract
Two inositol phospholipid-specific phospholipase C (PLC) isozymes (PLC-I and -II) have been purified from bovine brain. When PLC-I or PLC-II was microinjected (100-700 micrograms/ml) into quiescent NIH 3T3 cells, a time- and dose-dependent induction of DNA synthesis occurred, as demonstrated by [3H]thymidine incorporation into nuclear DNA. In addition, approximately to 8 hr after PLC injection, NIH 3T3 fibroblasts appeared spindle-shaped, refractile, and highly vacuolated, displaying a morphology similar to transformed cells. The morphologic transformation was apparent for 26-30 hr after which the injected cells reverted back to a normal phenotype. Microinjected PLC at a high concentration (1 mg/ml) was cytotoxic, dissolving the cytoplasmic membrane and leaving behind cellular ghosts. PLC is a key regulatory enzyme involved in cellular membrane signal transduction. Introduction of exogenous PLC into NIH 3T3 cells by microinjection induced a growth and oncogenic potential, as demonstrated by the ability of microinjected PLC (approximately 10,000 molecules per cell) to override the cellular G0 block, inducing DNA synthesis and morphologic transformation of growth-arrested fibroblast cells.
URI
https://scholarworks.unist.ac.kr/handle/201301/16462
URL
http://www.pnas.org/content/86/10/3659
DOI
10.1073/pnas.86.10.3659
ISSN
0027-8424
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BIO_Journal Papers
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