BROWSE

Related Researcher

Author's Photo

Suh, Pann-Ghill
BioSignal Network Lab (BSN)
Research Interests
  • Signal transduction, cancer, metabolism, phospholipase C

ITEM VIEW & DOWNLOAD

Phospholipase D1 is located and activated by protein kinase C alpha in the plasma membrane in 3Y1 fibroblast cell

Cited 38 times inthomson ciCited 36 times inthomson ci
Title
Phospholipase D1 is located and activated by protein kinase C alpha in the plasma membrane in 3Y1 fibroblast cell
Author
Kim, YongKim, Jung-EunLee, Sang DoLee, Taehoon G.Kim, Jae HoPark, Jong BaeHan, Jung MinJang, Sung KeySuh, Pann-GhillRyu, Sung Ho
Issue Date
1999-01
Publisher
ELSEVIER SCIENCE BV
Citation
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, v.1436, no.3, pp.319 - 330
Abstract
The subcellular location of phospholipase D1 (PLD1) and its activation by protein kinase Ca (PKC alpha) were examined by subcellular fractionation and by microscopic observation of green fluorescent protein-fused PLD1 (GFP-PLD1) or PKC alpha (GFP-PKC alpha) in fibroblastic 3Y1 cells. Major PLD1 immunoreactivity acid PKC alpha-stimulated PLD activity segregated with a plasma membrane marker, even though a significant amount was co-fractionated with markers for endoplasmic reticulum (ER) and Golgi. Upon treatment with phorbol myristate acetate (PMA), PKC alpha translocated from the cytosolic fraction to the membrane fraction to which PLD1 also localized. GFP-PLD1 was found in the plasma membrane as well as a in a perinuclear compartment consistent with ER and Golgi and in other dispersed vesicular structures in the cytoplasm, However, most of GFP-PKC alpha was translocated from the cytosol to the plasma membrane after treatment with PMA, From these results, we concluded that the plasma membrane is the major sire of PLD1 activation by PKCa in 3Y1 cells. (C) 1999 Elsevier Science B.V. All rights reserved
URI
https://scholarworks.unist.ac.kr/handle/201301/16456
URL
http://www.sciencedirect.com/science/article/pii/S0005276098001209
DOI
10.1016/S0005-2760(98)00120-9
ISSN
1388-1981
Appears in Collections:
BIO_Journal Papers
Files in This Item:
There are no files associated with this item.

find_unist can give you direct access to the published full text of this article. (UNISTARs only)

Show full item record

qrcode

  • mendeley

    citeulike

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

MENU