MR tracking of transplanted cells with "positive contrast" using manganese oxide nanoparticles
Cited 97 times inCited 97 times in
- MR tracking of transplanted cells with "positive contrast" using manganese oxide nanoparticles
- Gilad, Assaf A.; Walczak, Piotr; McMahon, Michael T.; Na, Hyon Bin; Lee, Jung Hee; An, Kwangjin; Hyeon, Taegwhan; Zijl, Peter C. M. van; Bulte, Jeff W. M.
- animal cell; animal tissue; article; cell population; cell proliferation; cell transplantation; cell viability; contrast enhancement; controlled study; cytotoxicity; diagnostic imaging; dose response; electroporation; glioma cell; hemisphere; in vivo study; low drug dose; nonhuman; nuclear magnetic resonance imaging; rat
- Issue Date
- MAGNETIC RESONANCE IN MEDICINE, v.60, no.1, pp.1 - 7
- Rat glioma cells were labeled using electroporation with either manganese oxide (MnO) or superparamagnetic iron oxide (SPIO) nanoparticles. The viability and proliferation of SPIO-labeled cells (1.9 mg Fe/ml) or cells electroporated with a low dose of MnO (100 mu g Mn/ml) was not significantly different from unlabeled cells; a higher MnO dose (785 mu g Mn/ml) was found to be toxic. The cellular ion content was 0.1-0.3 pg Mn/cell and 4.4 pg Fe/cell, respectively, with cellular relaxivities of 2.5-4.8 s(-1) (RI) and 45-84 s(-1) (R-2) for MnO-labeled cells. Labeled cells (SPIO and low-dose MnO) were each transplanted in contralateral brain hemispheres of rats and imaged in vivo at 9.4T. While SPIO-labeled cells produced a strong "negative contrast" due to the increase in R-2, MnO-Iabeled cells produced "positive contrast" with an increased R-1. Simultaneous imaging of both transplants with opposite contrast offers a method for MR "double labeling" of different cell populations.
- ; Go to Link
- Appears in Collections:
- ECHE_Journal Papers
- Files in This Item:
MRM 2008,60,1, MR tracking of transplanted cells with positive contrast using manganese oxide nanoparticles.pdf
can give you direct access to the published full text of this article. (UNISTARs only)
Show full item record
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.