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Rhee, Hyun-Woo
Biomimetic Chemistry Lab
Research Interests
  • Site-Specific Protein Labeling in Living Cells
  • Artificial Cofactors for Biomimetic Catalyst
  • Spatiotemporal Proteome mapping of living cells

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Selective fluorescent chemosensor for the bacterial alarmone (p)ppGpp

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Title
Selective fluorescent chemosensor for the bacterial alarmone (p)ppGpp
Other Titles
Selective fluorescent chemosensor for the bacterial alarmone (p)ppGpp
Author
Rhee, Hyun-WooLee, Chang-RoCho, Seung-HyonSong, Mi-RyungCashel, MichaelChoy, Hyon E.Seok, Young-JaeHong, Jong-In
Keywords
GUANOSINE 5'-DIPHOSPHATE 3'-DIPHOSPHATE; ESCHERICHIA-COLI; PYROPHOSPHATE SENSOR; TRANSCRIPTION
Issue Date
2008-01
Publisher
AMER CHEMICAL SOC
Citation
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, v.130, no.3, pp.784 - +
Abstract
We have developed the first selective fluorescent chemosensor (PyDPA) for (p)ppGpp, a bacterial and plant alarmone. By using pyrene-excimer fluorescence, PyDPA shows very good selectivity for (p)ppGpp from among other nucleotides in water. PyDPA was used for the real-time detection of in vitro ppGpp synthesis by bacterial ribosomal complexes
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DOI
10.1021/ja0759139
ISSN
0002-7863
Appears in Collections:
PHY_Journal Papers
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