Miniaturized probe based on a microelectromechanical system mirror for multiphoton microscopy
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- Miniaturized probe based on a microelectromechanical system mirror for multiphoton microscopy
- Jung, Woonggyu; Tang, Suo; McCormic, Daniel T.; Xie, Tiquiang; Ahn, Yeh-Chan; Su, Jianping; Tomov, Ivan V.; Krasieva, Tatiana B.; Tromberg, Bruce J.; Chen, Zhongping
- biological specimens; Double-clad; Fluorescent beads; Fluorescent signals; High collection efficiency; Micro-electro- mechanical system (MEMS); Microelectromechanical system (MEMS) mirror; Multiphoton microscopy (MPM); Optical society of America (OSA); Outer diameter (OD); Photonic crystal fiber (PCF); Preclinical studies; Probe based; Short pulses; size reductions
- Issue Date
- OPTICAL SOC AMER
- OPTICS LETTERS, v.33, no.12, pp.1324 - 1326
- A factor that limits the use of multiphoton microscopy (MFM) in clinical and preclinical studies is the lack of a compact and flexible probe. We report on a miniaturized MPM probe employing a microelectromechanical system (MEMS) scanning mirror and a double-clad photonic crystal fiber (DCPCF). The use of a MEMS mirror and a DCPCF provides many advantages, such as size reduction, rapid and precise scanning, efficient delivery of short pulses, and high collection efficiency of fluorescent signals. The completed probe was 1 cm in outer diameter and 14 cm in length. The developed probe was integrated into an MPM system and used to image fluorescent beads, paper, and biological specimens.
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