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Takayama, Shuichi
Cell and Microfluidics Lab
Research Interests
  • Bio-MEMS and Microfluidics
  • Bio-Nanotechnology
  • Biofluids
  • Biomaterials
  • Tissue Engineering and Regenerative Medicine.

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Media additives to promote spheroid circularity and compactness in hanging drop platform

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Title
Media additives to promote spheroid circularity and compactness in hanging drop platform
Author
Leung, Brendan M.Lesher-Perez, Sasha CaiMatsuoka, ToshikiMoraes, ChristopherTakayama, Shuichi
Issue Date
2015-02
Publisher
Royal Society of Chemistry
Citation
BIOMATERIALS SCIENCE, v.3, no.2, pp.336 - 344
Abstract
Three-dimensional spheroid cultures have become increasingly popular as drug screening platforms, especially with the advent of different high throughput spheroid forming technologies. However, comparing drug efficacy across different cell types in spheroid culture can be difficult due to variations in spheroid morphologies and transport characteristics. Improving the reproducibility of compact, circular spheroids contributes to standardizing and increasing the fidelity of the desired gradient profiles in these drug screening three-dimensional tissue cultures. In this study we discuss the role that circularity and compaction has on spheroids, and demonstrate the impact methylcellulose (MethoCel) and collagen additives in the culture media can contribute to more compact and circular spheroid morphology. We demonstrate that improved spheroid formation is not a simple function of increased viscosity of the different macromolecule additives, suggesting that other macromolecular characteristics contribute to improved spheroid formation. Of the various macromolecular additives tested for hanging drop culture, MethoCel provided the most desirable spheroid formation. Additionally, the higher viscosity of MethoCel-containing media improved the ease of imaging of cellular spheroids within hanging drop cultures by reducing motion-induced image blur.
URI
https://scholarworks.unist.ac.kr/handle/201301/10621
URL
http://pubs.rsc.org/en/Content/ArticleLanding/2015/BM/C4BM00319E#!divAbstract
DOI
10.1039/c4bm00319e
ISSN
2047-4830
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