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Suh, Pann-Ghill
BioSignal Network Lab (BSN)
Research Interests
  • Signal transduction, cancer, metabolism, phospholipase C

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O-GlcNAc transferase is activated by CaMKIV-dependent phosphorylation under potassium chloride-induced depolarization in NG-108-15 cells

Cited 27 times inthomson ciCited 29 times inthomson ci
Title
O-GlcNAc transferase is activated by CaMKIV-dependent phosphorylation under potassium chloride-induced depolarization in NG-108-15 cells
Author
Song, MinseokKim, Hyeon-SooParka, Ji-ManKim, Sun-HeeKim, In-HooRyu, Sung HoSuh, Pann-Ghill
Issue Date
2008-01
Publisher
ELSEVIER SCIENCE INC
Citation
CELLULAR SIGNALLING, v.20, no.1, pp.94 - 104
Abstract
Post-translational modification of cellular proteins by beta-o-linked N-acetylglucosamine (o-GlcNAc) moieties plays a significant role in signal transduction by modulating protein stability, protein-protein interactions, transactivation processes, and the enzyme activities of target proteins. Though various classes of proteins are known to be regulated by o-GlcNAc modification (o-GlcNAcylation), the mechanism that regulates o-linked GlcNAc transferase (OGT) activity remains unknown. Here, we report that potassium chloride-induced depolarization provokes the activation of OGT and subsequent o-GlcNAcylation of proteins in neuroblastoma NG-108-15 cells. Moreover, such an induction of protein o-GlcNAcylation was abolished by treating cells with either a voltage-gated calcium channel inhibitor or a calcium/calmodulin-dependent protein kinase (CaMK) inhibitor. In addition, CaMKIV was found to specifically phosphorylate and activate OGT in vivo and in vitro, which implies that CaMKIV is required for depolarization-induced activation of OGT. Furthermore, we found that OGT is involved in depolarization-induced and CaMKIV-dependent activation of activator protein-1 (AP-1) and subsequent tissue inhibitor of metalloproteinase-1 (Timp-1) gene expression. Taken together, our findings suggest that CaMKIV activated OGT, and OGT has an essential role on the process of CaMKIV-dependent AP-1 activation under depolarization in neuronal cells. (C) 2007 Elsevier Inc. All rights reserved.
URI
https://scholarworks.unist.ac.kr/handle/201301/10100
URL
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=36349032408
DOI
10.1016/j.cellsig.2007.09.002
ISSN
0898-6568
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BIO_Journal Papers
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