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- Park, Jongnam
- Materials and Chemistry Lab.
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Ni/NiO core/shell nanoparticles for selective binding and magnetic separation of histidine-tagged proteins
- Author(s)
- Lee, In Su, Lee, Nohyun, Park, Jongnam, Kim, Byung Hyo, Yi, Yong-Weon, Kim, Taeuk, Kim, Tae Kook, Lee, In Hwan, Paik, Seung R., Hyeon, Taeghwan
- Issued Date
- 2006-08
- Citation
- JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, v.128, no.33, pp.10658 - 10659
- Abstract
- Ni/NiO core/shell nanoparticles having high affinity with polyhistidine were synthesized by decomposition of a Ni surfactant complex followed by air oxidation. Ni/NiO nanoparticles showed selective and efficient binding to histidine-tagged proteins and easy separation by using a magnet. These provided a more convenient way to efficient purification of histidine-tagged proteins compared with the conventional Ni-NTA complex-bound resins and microbeads.
- Publisher
- AMER CHEMICAL SOC
- ISSN
- 0002-7863
- Keyword
- MONODISPERSE, SUPERLATTICES, NANOCRYSTALS, MOLECULES, CELLS
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