BROWSE

Related Researcher

Author

Cho, Moo Je
Ulsan National Institute of Science and Technology
Research Interests
  • Calcium Signaling

ITEM VIEW & DOWNLOAD

ISOLATION OF 2 SOYBEAN G-BOX BINDING-FACTORS WHICH INTERACT WITH A G-BOX SEQUENCE OF AN AUXIN-RESPONSIVE GENE

Cited 63 times inthomson ciCited 43 times inthomson ci
Title
ISOLATION OF 2 SOYBEAN G-BOX BINDING-FACTORS WHICH INTERACT WITH A G-BOX SEQUENCE OF AN AUXIN-RESPONSIVE GENE
Author
HONG, JCCHEONG, YHNAGAO, RTBAHK, JDKEY, JLCho, Moo Je
Keywords
CHALCONE SYNTHASE PROMOTER; LEUCINE ZIPPER PROTEINS; DNA-BINDING; REGULATED GENE; COILED COIL; EXPRESSION; TRANSCRIPTION; SPECIFICITY; RECOGNITION; HYPOCOTYL
Issue Date
199508
Publisher
WILEY-BLACKWELL
Citation
PLANT JOURNAL, v.8, no.2, pp.199 - 211
Abstract
G-box binding factors (GBFs) constitute a family of plant DNA-binding proteins that bind to the G-box motif, a regulatory cis element present in many plant genes with a palindromic DNA motif of CACGTG. Previously TCCACGTGTC, a G-box motif, from an auxin responsive gene GmAux28 has been identified as a sequence-specific protein-binding site. Here the isolation of two soybean cDNA clones, referred to as SGBF-1 and SGBF-2, encoding proteins which bind to the G-box motif is reported. The primary structure of SGBF-1 and SGBF-2 predicts that these proteins contain a basic leucine zipper (bZIP) DNA-binding domain and an N-terminal proline-rich domain. A dramatic difference in the pattern of protein-DNA complex formation was observed when recombinant SGBF-1 and SGBF-2 proteins were analyzed by electrophoretic mobility shift assays (EMSAs). The SGBF-1 binding pattern obtained with the G-box probe resulted in three major retarded bands while the SGBF-2 formed a single complex. This shows that the characteristically diffuse banding pattern of plant nuclear proteins interacting with the G-box is also observed in a binding assay using only one recombinant GBF. EMSAs were performed with a few selected binding sequences to study the effect of flanking nucleotides to the hexanucleotide G-box core motif. The binding specificity of the SGBF proteins resembles that described for type A cauliflower nuclear G-box binding proteins which bind class I G-box elements [((G)/(T))((C)/(A))CACGTG((G)/(T)) ((A)/(C))]. Phylogenetic analysis of 13 GBF-like proteins from various plant species reveals that the SGBF-1 and SGBF-2 proteins belong to different lineages, suggesting that they may have distinct functions in activating transcription.
URI
Go to Link
DOI
http://dx.doi.org/10.1046/j.1365-313X.1995.08020199.x
ISSN
0960-7412
Appears in Collections:
SLS_Journal Papers

find_unist can give you direct access to the published full text of this article. (UNISTARs only)

Show full item record

qr_code

  • mendeley

    citeulike

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

MENU