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DC Field | Value | Language |
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dc.citation.endPage | 211 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 199 | - |
dc.citation.title | PLANT JOURNAL | - |
dc.citation.volume | 8 | - |
dc.contributor.author | HONG, JC | - |
dc.contributor.author | CHEONG, YH | - |
dc.contributor.author | NAGAO, RT | - |
dc.contributor.author | BAHK, JD | - |
dc.contributor.author | KEY, JL | - |
dc.contributor.author | Cho, Moo Je | - |
dc.date.accessioned | 2023-12-22T12:41:31Z | - |
dc.date.available | 2023-12-22T12:41:31Z | - |
dc.date.created | 2014-09-19 | - |
dc.date.issued | 1995-08 | - |
dc.description.abstract | G-box binding factors (GBFs) constitute a family of plant DNA-binding proteins that bind to the G-box motif, a regulatory cis element present in many plant genes with a palindromic DNA motif of CACGTG. Previously TCCACGTGTC, a G-box motif, from an auxin responsive gene GmAux28 has been identified as a sequence-specific protein-binding site. Here the isolation of two soybean cDNA clones, referred to as SGBF-1 and SGBF-2, encoding proteins which bind to the G-box motif is reported. The primary structure of SGBF-1 and SGBF-2 predicts that these proteins contain a basic leucine zipper (bZIP) DNA-binding domain and an N-terminal proline-rich domain. A dramatic difference in the pattern of protein-DNA complex formation was observed when recombinant SGBF-1 and SGBF-2 proteins were analyzed by electrophoretic mobility shift assays (EMSAs). The SGBF-1 binding pattern obtained with the G-box probe resulted in three major retarded bands while the SGBF-2 formed a single complex. This shows that the characteristically diffuse banding pattern of plant nuclear proteins interacting with the G-box is also observed in a binding assay using only one recombinant GBF. EMSAs were performed with a few selected binding sequences to study the effect of flanking nucleotides to the hexanucleotide G-box core motif. The binding specificity of the SGBF proteins resembles that described for type A cauliflower nuclear G-box binding proteins which bind class I G-box elements [((G)/(T))((C)/(A))CACGTG((G)/(T)) ((A)/(C))]. Phylogenetic analysis of 13 GBF-like proteins from various plant species reveals that the SGBF-1 and SGBF-2 proteins belong to different lineages, suggesting that they may have distinct functions in activating transcription. |
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dc.identifier.bibliographicCitation | PLANT JOURNAL, v.8, no.2, pp.199 - 211 | - |
dc.identifier.doi | 10.1046/j.1365-313X.1995.08020199.x | - |
dc.identifier.issn | 0960-7412 | - |
dc.identifier.scopusid | 2-s2.0-0029347042 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/6383 | - |
dc.identifier.url | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0029347042 | - |
dc.identifier.wosid | A1995RP94800004 | - |
dc.language | 영어 | - |
dc.publisher | WILEY-BLACKWELL | - |
dc.title | ISOLATION OF 2 SOYBEAN G-BOX BINDING-FACTORS WHICH INTERACT WITH A G-BOX SEQUENCE OF AN AUXIN-RESPONSIVE GENE | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scopus | - |
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