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Author

Cho, Yoon-Kyoung
Integrated Nano-Biotech Lab (INBL)
Research Interests
  • Microfluidics, Lab-on-a-chip, personalized biomedical diagnostics, nanobioengineering

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World-to-chip microfluidic interface with built-in valves for multichamber chip-based PCR assays

Cited 35 times inthomson ciCited 41 times inthomson ci
Title
World-to-chip microfluidic interface with built-in valves for multichamber chip-based PCR assays
Author
Oh, KWPark, CSNamkoong, KKim, JOck, KSKim, SKim, YACho, Yoon-KyoungKo, C
Keywords
DNA ANALYSIS DEVICE; CAPILLARY-ELECTROPHORESIS; SAMPLE PREPARATION; MICROCHIP PCR; SYSTEMS; INTERCONNECTS; AMPLIFICATION; DIAGNOSTICS; TEMPERATURE; MICROSYSTEM
Issue Date
2005
Publisher
ROYAL SOC CHEMISTRY
Citation
LAB ON A CHIP, v.5, no.8, pp.845 - 850
Abstract
We report a practical world-to-chip microfluidic interfacing method with built-in valves suitable for microscale multichamber chip-based assays. One of the primary challenges associated with the successful commercialization of fully integrated microfluidic systems has been the lack of reliable world-to-chip microfluidic interconnections. After sample loading and sealing, leakage tests were conducted at 100 °C for 30 min and no detectable leakage flows were found during the test for 100 microchambers. To demonstrate the utility of our world-to-chip microfluidic interface, we designed a microscale PCR chip with four chambers and performed PCR assays. The PCR results yielded a 100% success rate with no contamination or leakage failures. In conclusion, we have introduced a simple and inexpensive microfluidic interfacing system for both sample loading and sealing with no dead volume, no leakage flow and biochemical compatibility.
URI
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DOI
http://dx.doi.org/10.1039/b503437j
ISSN
1473-0197
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