World-to-chip microfluidic interface with built-in valves for multichamber chip-based PCR assays
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- World-to-chip microfluidic interface with built-in valves for multichamber chip-based PCR assays
- Oh, KW; Park, CS; Namkoong, K; Kim, J; Ock, KS; Kim, S; Kim, YA; Cho, Yoon-Kyoung; Ko, C
- DNA ANALYSIS DEVICE; CAPILLARY-ELECTROPHORESIS; SAMPLE PREPARATION; MICROCHIP PCR; SYSTEMS; INTERCONNECTS; AMPLIFICATION; DIAGNOSTICS; TEMPERATURE; MICROSYSTEM
- Issue Date
- ROYAL SOC CHEMISTRY
- LAB ON A CHIP, v.5, no.8, pp.845 - 850
- We report a practical world-to-chip microfluidic interfacing method with built-in valves suitable for microscale multichamber chip-based assays. One of the primary challenges associated with the successful commercialization of fully integrated microfluidic systems has been the lack of reliable world-to-chip microfluidic interconnections. After sample loading and sealing, leakage tests were conducted at 100 °C for 30 min and no detectable leakage flows were found during the test for 100 microchambers. To demonstrate the utility of our world-to-chip microfluidic interface, we designed a microscale PCR chip with four chambers and performed PCR assays. The PCR results yielded a 100% success rate with no contamination or leakage failures. In conclusion, we have introduced a simple and inexpensive microfluidic interfacing system for both sample loading and sealing with no dead volume, no leakage flow and biochemical compatibility.
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