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- Suh, Pann-Ghill
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PURIFICATION AND SOME PROPERTIES OF A PHOSPHOLIPASE-A2 FROM BOVINE PLATELETS
- Author(s)
- KIM, DK, Suh, Pann-Ghill, RYU, SH
- Issued Date
- 1991-01
- Citation
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.174, no.1, pp.189 - 196
- Abstract
- An intracellular form of phospholipase A2 was purified about 47,500-fold to near homogeneity from bovine platelets 100,000 × g supernatant by sequential use of column chromatographies on Heparin-Sepharose, DEAE-Sephacel, Butyl-Toyopearl, Sephacryl S-300, DEAE-5PW HPLC, TSK G 3000 SW HPLC and Mono Q FPLC. The final preparation showed a single band on SDS-polyacrylamide gel, and its molecular mass was estimated to be approximately 100,000 daltons. The purified PLA2 showed maximal activity at alkaline pH(pH 9.0-10.0) and considerable activity at 0.3-1.0μM calcium concentration. It hydrolyzed phosphatidylcholine containing arachidonate at sn-2 position with high selectivity in comparison to linoleate.
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- ISSN
- 0006-291X
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