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Suh, Pann-Ghill
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dc.citation.endPage 196 -
dc.citation.number 1 -
dc.citation.startPage 189 -
dc.citation.title BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS -
dc.citation.volume 174 -
dc.contributor.author KIM, DK -
dc.contributor.author Suh, Pann-Ghill -
dc.contributor.author RYU, SH -
dc.date.accessioned 2023-12-22T13:08:46Z -
dc.date.available 2023-12-22T13:08:46Z -
dc.date.created 2014-09-03 -
dc.date.issued 1991-01 -
dc.description.abstract An intracellular form of phospholipase A2 was purified about 47,500-fold to near homogeneity from bovine platelets 100,000 × g supernatant by sequential use of column chromatographies on Heparin-Sepharose, DEAE-Sephacel, Butyl-Toyopearl, Sephacryl S-300, DEAE-5PW HPLC, TSK G 3000 SW HPLC and Mono Q FPLC. The final preparation showed a single band on SDS-polyacrylamide gel, and its molecular mass was estimated to be approximately 100,000 daltons. The purified PLA2 showed maximal activity at alkaline pH(pH 9.0-10.0) and considerable activity at 0.3-1.0μM calcium concentration. It hydrolyzed phosphatidylcholine containing arachidonate at sn-2 position with high selectivity in comparison to linoleate. -
dc.identifier.bibliographicCitation BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.174, no.1, pp.189 - 196 -
dc.identifier.doi 10.1016/0006-291X(91)90504-Z -
dc.identifier.issn 0006-291X -
dc.identifier.scopusid 2-s2.0-0025963999 -
dc.identifier.uri https://scholarworks.unist.ac.kr/handle/201301/5716 -
dc.identifier.url http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0025963999 -
dc.identifier.wosid A1991ET20700030 -
dc.language 영어 -
dc.publisher ACADEMIC PRESS INC ELSEVIER SCIENCE -
dc.title PURIFICATION AND SOME PROPERTIES OF A PHOSPHOLIPASE-A2 FROM BOVINE PLATELETS -
dc.type Article -
dc.description.journalRegisteredClass scopus -

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