BROWSE

Related Researcher

Author

Suh, Pann-Ghill
BioSignal Network Lab (BSN)
Research Interests
  • Signal transduction, cancer, metabolism, phospholipase C

ITEM VIEW & DOWNLOAD

O-GlcNAc modification modulates the expression of osteocalcin via OSE2 and Runx2

Cited 9 times inthomson ciCited 10 times inthomson ci
Title
O-GlcNAc modification modulates the expression of osteocalcin via OSE2 and Runx2
Author
Kim, Sun-HeeKim, Yun-HeeSong, MinseokAn, Sang HeeByun, Ha-YoungHeo, KyunLim, SeyoungOh, Young-SeokRyu, Sung HoSuh, Pann-Ghill
Keywords
O-GlcNAc; OSE2; Osteoblast; Osteocalcin; Runx2
Issue Date
200710
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Citation
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.362, no.2, pp.325 - 329
Abstract
O-Linked β-N-acetylglucosamine (O-GlcNAc) modification, a reversible post-translational modification, has been implicated in the regulation of protein stability, subcellular localization of proteins and protein-protein interaction. Here, we demonstrate that O-GlcNAc modification regulates the expression of osteocalcin, an osteoblast-specific marker, via Runx2 transcriptional activity in osteoblastic differentiation. Protein-associated O-GlcNAc was increased during osteoblastic differentiation in MC3T3-E1 preosteoblasts. In addition, PUGNAc, an inhibitor of O-GlcNAcase, potentiated the expression of osteocalcin caused by ascorbic acid, parathyroid hormone (PTH) and forskolin. By conducting activity assays of the osteocalcin promoter and transcription factor, we found that the OSE2 site in the osteocalcin promoter and Runx2 were important for increased osteocalcin promoter activity by PUGNAc. Furthermore, PUGNAc led to increased O-GlcNAc modification of Runx2, which regulated the transcription of its target gene osteocalcin. Thus, these data provide evidence that O-GlcNAc modification may be a new mode of osteoblastic differentiation regulation.
URI
Go to Link
DOI
http://dx.doi.org/10.1016/j.bbrc.2007.07.149
ISSN
0006-291X
Appears in Collections:
SLS_Journal Papers

find_unist can give you direct access to the published full text of this article. (UNISTARs only)

Show full item record

qr_code

  • mendeley

    citeulike

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

MENU