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Author

Kwon, H. Moo
Inflammation and Kidney Disorder Lab
Research Interests
  • TonEBP, Obesity, Cancer, Chronic inflammatory diseases, Brain disorder, Kidney disorders, Genomic instability

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RENAL NA-MYO-INOSITOL COTRANSPORTER MESSENGER-RNA EXPRESSION IN XENOPUS-OOCYTES - REGULATION BY HYPERTONICITY

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Title
RENAL NA-MYO-INOSITOL COTRANSPORTER MESSENGER-RNA EXPRESSION IN XENOPUS-OOCYTES - REGULATION BY HYPERTONICITY
Author
Kwon, H. MooYAMAUCHI, AUCHIDA, SROBEY, RBGARCIAPEREZ, ABURG, MBHANDLER, JS
Keywords
Cytochalasin B;  Madin-Darby canine kidney cells;  Osmolytes;  Phloretin;  Phlorizin
Issue Date
199102
Publisher
American Physiological Society
Citation
AMERICAN JOURNAL OF PHYSIOLOGY, v.260, no.2, pp.F258 - F263
Abstract
Canine renal cells in culture (MDCK cells) accumulate organic osmolytes, including myo-inositol (MI), in response to hypertonic stress. When medium tonicity is increased, intracellular concentration of MI rises because hypertonicity elicits increased uptake of MI via Na-MI cotransporter(s). To study the mechanism for this increase in cotransporter activity, poly(A)+ RNA isolated from MDCK cells maintained in hypertonic or isotonic medium was injected into Xenopus oocytes, and Na-dependent MI uptake was measured 3-5 days later. Poly(A)+ RNA from hypertonic cells induced clear expression of the cotransporter. In contrast, oocytes injected with poly(A)+ RNA isolated from MDCK cells maintained in isotonic medium exhibited cotransporter activity like oocytes injected with water. Upon size fractionation of RNA, peak activity appeared in a fraction that contained poly(A)+ RNA with median size of ~4 kilobases. Na-dependent MI uptake by poly(A)+ RNA-injected oocytes was inhibited by both phlorizin and phloretin. We suggest that hypertonicity-induced upregulation of the Na-MI cotransporter involves an increase in mRNA and synthesis of cotransporter protein(s).
URI
http://scholarworks.unist.ac.kr/handle/201301/4919
ISSN
0002-9513
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