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Kwon, H. Moo
Inflammation and Kidney Disorder Lab
Research Interests
  • TonEBP, Obesity, Cancer, Chronic inflammatory diseases, Brain disorder, Kidney disorders, Genomic instability

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Silencing of TonEBP/NFAT5 transcriptional activator by RNA interference

Cited 40 times inthomson ciCited 41 times inthomson ci
Title
Silencing of TonEBP/NFAT5 transcriptional activator by RNA interference
Author
Na, KYWoo, SKLee, SDKwon, H. Moo
Keywords
ENHANCER-BINDING PROTEIN; GENE-TRANSCRIPTION; TONICITY; NFAT
Issue Date
200302
Publisher
AMER SOC NEPHROLOGY
Citation
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, v.14, no.2, pp.283 - 288
Abstract
TonEBP is a transcriptional activator that is expressed throughout development in many tissues and cell types. In the kidney medulla, TonEBP appears to be an important local regulator of differentiation by virtue of stimulating several genes. To study the function of TonEBP, two small interfering RNA (siRNA) duplexes were developed that reduced TonEBP expression effectively via RNA interference. The silencing lasted only 3 d after introduction of the TonEBP-siRNA's. As expected, TonEBP-driven reporter gene expression and expression of the sodium/myo-inositol cotransproter (SMIT), aldose reductase (AR) and heat shock protein 70 (HSP70) mRNA were significantly decreased in cells where TonEBP expression was silenced. These data provide direct evidence that the SMIT, AR, and HSP70 genes are targets of TonEBP, although the potential role of other proteins, such as accessory proteins, cannot be excluded. The TonEBP-siRNA is an effective tool that should prove useful in the investigation of loss-of-function relationship in cells.
URI
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DOI
http://dx.doi.org/10.1097/01.ASN.0000045050.19544.B2
ISSN
1046-6673
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