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Author

Takayama, Shuichi
Cell and Microfluidics Lab
Research Interests
  • Bio-MEMS and Microfluidics

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Micro-ring structures stabilize microdroplets to enable long term spheroid culture in 384 hanging drop array plates

Cited 11 times inthomson ciCited 8 times inthomson ci
Title
Micro-ring structures stabilize microdroplets to enable long term spheroid culture in 384 hanging drop array plates
Author
Hsiao, Amy Y.Tung, Yi-ChungKuo, Chuan-HsienMosadegh, BobakBedenis, RachelPienta, Kenneth J.Takayama, Shuichi
Keywords
3-D cell culture; 3D culture; Cell analysis; Cell constructs; Cell spheroids; Chemical perturbations; Droplet arrays; Droplet spreading; Droplet stability; Drug screening; High-throughput; Mass production; Mechanical perturbations; Mechanical shock; Micro droplets; Micro-scales; Microliters; Microrings; Spheroid; Structural variations; Through hole
Issue Date
201204
Publisher
SPRINGER
Citation
BIOMEDICAL MICRODEVICES, v.14, no.2, pp.313 - 323
Abstract
Using stereolithography, 20 different structural variations comprised of millimeter diameter holes surrounded by trenches, plateaus, or micro-ring structures were prepared and tested for their ability to stably hold arrays of microliter sized droplets within the structures over an extended period of time. The micro-ring structures were the most effective in stabilizing droplets against mechanical and chemical perturbations. After confirming the importance of micro-ring structures using rapid prototyping, we developed an injection molding tool for mass production of polystyrene 3D cell culture plates with an array of 384 such micro-ring surrounded through-hole structures. These newly designed and injection molded polystyrene 384 hanging drop array plates with micro-rings were stable and robust against mechanical perturbations as well as surface fouling-facilitated droplet spreading making them capable of long term cell spheroid culture of up to 22 days within the droplet array. This is a significant improvement over previously reported 384 hanging drop array plates which are susceptible to small mechanical shocks and could not reliably maintain hanging drops for longer than a few days. With enhanced droplet stability, the hanging drop array plates with micro-ring structures provide better platforms and open up new opportunities for high-throughput preparation of microscale 3D cell constructs for drug screening and cell analysis
URI
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DOI
http://dx.doi.org/10.1007/s10544-011-9608-5
ISSN
1387-2176
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