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DC Field | Value | Language |
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dc.citation.endPage | 323 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 313 | - |
dc.citation.title | BIOMEDICAL MICRODEVICES | - |
dc.citation.volume | 14 | - |
dc.contributor.author | Hsiao, Amy Y. | - |
dc.contributor.author | Tung, Yi-Chung | - |
dc.contributor.author | Kuo, Chuan-Hsien | - |
dc.contributor.author | Mosadegh, Bobak | - |
dc.contributor.author | Bedenis, Rachel | - |
dc.contributor.author | Pienta, Kenneth J. | - |
dc.contributor.author | Takayama, Shuichi | - |
dc.date.accessioned | 2023-12-22T05:13:07Z | - |
dc.date.available | 2023-12-22T05:13:07Z | - |
dc.date.created | 2013-06-20 | - |
dc.date.issued | 2012-04 | - |
dc.description.abstract | Using stereolithography, 20 different structural variations comprised of millimeter diameter holes surrounded by trenches, plateaus, or micro-ring structures were prepared and tested for their ability to stably hold arrays of microliter sized droplets within the structures over an extended period of time. The micro-ring structures were the most effective in stabilizing droplets against mechanical and chemical perturbations. After confirming the importance of micro-ring structures using rapid prototyping, we developed an injection molding tool for mass production of polystyrene 3D cell culture plates with an array of 384 such micro-ring surrounded through-hole structures. These newly designed and injection molded polystyrene 384 hanging drop array plates with micro-rings were stable and robust against mechanical perturbations as well as surface fouling-facilitated droplet spreading making them capable of long term cell spheroid culture of up to 22 days within the droplet array. This is a significant improvement over previously reported 384 hanging drop array plates which are susceptible to small mechanical shocks and could not reliably maintain hanging drops for longer than a few days. With enhanced droplet stability, the hanging drop array plates with micro-ring structures provide better platforms and open up new opportunities for high-throughput preparation of microscale 3D cell constructs for drug screening and cell analysis | - |
dc.identifier.bibliographicCitation | BIOMEDICAL MICRODEVICES, v.14, no.2, pp.313 - 323 | - |
dc.identifier.doi | 10.1007/s10544-011-9608-5 | - |
dc.identifier.issn | 1387-2176 | - |
dc.identifier.scopusid | 2-s2.0-84861460487 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/4058 | - |
dc.identifier.url | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84861460487 | - |
dc.identifier.wosid | 000301544500007 | - |
dc.language | 영어 | - |
dc.publisher | SPRINGER | - |
dc.title | Micro-ring structures stabilize microdroplets to enable long term spheroid culture in 384 hanging drop array plates | - |
dc.type | Article | - |
dc.relation.journalWebOfScienceCategory | Engineering, Biomedical; Nanoscience & Nanotechnology | - |
dc.relation.journalResearchArea | Engineering; Science & Technology - Other Topics | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | Micro-ring | - |
dc.subject.keywordAuthor | Spheroid | - |
dc.subject.keywordAuthor | High-throughput | - |
dc.subject.keywordAuthor | 3D culture | - |
dc.subject.keywordAuthor | Hanging drop | - |
dc.subject.keywordAuthor | Stereolithography | - |
dc.subject.keywordPlus | STEREOLITHOGRAPHY TECHNIQUE | - |
dc.subject.keywordPlus | TEMPORAL BONE | - |
dc.subject.keywordPlus | SURGERY | - |
dc.subject.keywordPlus | POLYMERIZATION | - |
dc.subject.keywordPlus | MICROMACHINES | - |
dc.subject.keywordPlus | MECHANISMS | - |
dc.subject.keywordPlus | SCAFFOLDS | - |
dc.subject.keywordPlus | MODEL | - |
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