BROWSE

Related Researcher

Author

Takayama, Shuichi
Cell and Microfluidics Lab
Research Interests
  • Bio-MEMS and Microfluidics

ITEM VIEW & DOWNLOAD

Microprinted Feeder Cells Guide Embryonic Stem Cell Fate

Cited 0 times inthomson ciCited 9 times inthomson ci
Title
Microprinted Feeder Cells Guide Embryonic Stem Cell Fate
Author
Tavana, HosseinMosadegh, BobakZamankhan, ParsaGrotberg, James B.Takayama, Shuichi
Keywords
Aqueous two phase system; Cell printing; Cell-cell contact; Embryonic stem cells; Microenvironments
Issue Date
201110
Publisher
WILEY-BLACKWELL
Citation
BIOTECHNOLOGY AND BIOENGINEERING, v.108, no.10, pp.2509 - 2516
Abstract
We introduce a non-contact approach to microprint multiple types of feeder cells in a microarray format using immiscible aqueous solutions of two biopolymers. Droplets of cell suspension in the denser aqueous phase are printed on a substrate residing within a bath of the immersion aqueous phase. Due to their affinity to the denser phase, cells remain localized within the drops and adhere to regions of the substrate underneath the drops. We show the utility of this technology for creating duplex heterocellular stem cell niches by printing two different support cell types on a gel surface and overlaying them with mouse embryonic stem cells (mESCs). As desired, the type of printed support cell spatially direct the fate of overlaid mESCs. Interestingly, we found that interspaced mESCs colonies on differentiation-inducing feeder cells show enhanced neuronal differentiation and give rise to dense networks of neurons. This cell printing technology provides unprecedented capabilities to efficiently identify the role of various feeder cells in guiding the fate of stem cells
URI
Go to Link
DOI
http://dx.doi.org/10.1002/bit.23190
ISSN
0006-3592
Appears in Collections:
SLS_Journal Papers

find_unist can give you direct access to the published full text of this article. (UNISTARs only)

Show full item record

qr_code

  • mendeley

    citeulike

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

MENU