Engineering Escherichia coli for efficient cellobiose utilization
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- Engineering Escherichia coli for efficient cellobiose utilization
- Vinuselvi, Parisutham; Lee, Sung Kuk
- A-carbon; Asc operon; Cellobiose; Chb operon; Constitutive promoters; E. coli; Energy source; Evolutionary adaptation; Hydrolyze cellulose; Selection pressures; Simultaneous saccharification and fermentation
- Issue Date
- APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, v.92, no.1, pp.125 - 132
- Escherichia coli normally cannot utilize the beta-glucoside sugar cellobiose as a carbon and energy source unless a stringent selection pressure for survival is present. The cellobiose-utilization phenotype can be conferred by mutations in the two cryptic operons, chb and asc. In this study, the cellobiose-utilization phenotype was conferred to E. coli by replacing the cryptic promoters of these endogenous operons with a constitutive promoter. Evolutionary adaptation of the engineered strain CP12CHBASC by repeated subculture in cellobiose-containing minimal medium led to an increase in the rate of cellobiose uptake and cell growth on cellobiose. An efficient cellobiose-metabolizing E. coli strain would be of great importance over glucose-metabolizing E. coli for a simultaneous saccharification and fermentation process, as the cost of the process would be reduced by eliminating one of the three enzymes needed to hydrolyze cellulose into simple sugars.
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