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DC Field | Value | Language |
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dc.citation.endPage | 132 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 125 | - |
dc.citation.title | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.volume | 92 | - |
dc.contributor.author | Vinuselvi, Parisutham | - |
dc.contributor.author | Lee, Sung Kuk | - |
dc.date.accessioned | 2023-12-22T05:44:38Z | - |
dc.date.available | 2023-12-22T05:44:38Z | - |
dc.date.created | 2013-06-17 | - |
dc.date.issued | 2011-10 | - |
dc.description.abstract | Escherichia coli normally cannot utilize the beta-glucoside sugar cellobiose as a carbon and energy source unless a stringent selection pressure for survival is present. The cellobiose-utilization phenotype can be conferred by mutations in the two cryptic operons, chb and asc. In this study, the cellobiose-utilization phenotype was conferred to E. coli by replacing the cryptic promoters of these endogenous operons with a constitutive promoter. Evolutionary adaptation of the engineered strain CP12CHBASC by repeated subculture in cellobiose-containing minimal medium led to an increase in the rate of cellobiose uptake and cell growth on cellobiose. An efficient cellobiose-metabolizing E. coli strain would be of great importance over glucose-metabolizing E. coli for a simultaneous saccharification and fermentation process, as the cost of the process would be reduced by eliminating one of the three enzymes needed to hydrolyze cellulose into simple sugars. | - |
dc.identifier.bibliographicCitation | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, v.92, no.1, pp.125 - 132 | - |
dc.identifier.doi | 10.1007/s00253-011-3434-9 | - |
dc.identifier.issn | 0175-7598 | - |
dc.identifier.scopusid | 2-s2.0-82455167867 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/3531 | - |
dc.identifier.url | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=82455167867 | - |
dc.identifier.wosid | 000294821200012 | - |
dc.language | 영어 | - |
dc.publisher | SPRINGER | - |
dc.title | Engineering Escherichia coli for efficient cellobiose utilization | - |
dc.type | Article | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | chb operon | - |
dc.subject.keywordAuthor | Cellobiose metabolism | - |
dc.subject.keywordAuthor | asc operon | - |
dc.subject.keywordAuthor | Cryptic genes | - |
dc.subject.keywordAuthor | Escherichia coli | - |
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