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Author

Cho, Yoon-Kyoung
Integrated Nano-Biotech Lab (INBL)
Research Interests
  • Microfluidics, Lab-on-a-chip, personalized biomedical diagnostics, nanobioengineering

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Lab-on-a-Disc for Simultaneous Determination of Nutrients in Water

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Title
Lab-on-a-Disc for Simultaneous Determination of Nutrients in Water
Author
Hwang, HyundooKim, YubinCho, JuhyeLee, Ji-yoonChoi, Man-SikCho, Yoon-Kyoung
Keywords
Analysis time; Centrifugal Forces; Centrifugal microfluidics; Certified reference materials; Colorimetric detection; Fluid transfer; Fully automated; Liquid sample; Micro valves; Nutrient concentrations; Parallel process; Rotating disc; Simultaneous determinations; Water samples
Issue Date
201303
Publisher
AMER CHEMICAL SOC
Citation
ANALYTICAL CHEMISTRY, v.85, no.5, pp.2954 - 2960
Abstract
In this study, we describe a novel platform based on centrifugal microfluidics for simultaneous determination of nitrite, nitrate and nitrite, ammonium, orthophosphate, and silicate in water samples. All processes from sample metering to detection were integrated and automatically conducted on a rotating disc-shaped device. Fluid transfer was controlled by laser irradiation on the ferrowax-based microvalves. Liquid samples and reagents were pumped by centrifugal force in the rotating disc, and their positions and movements were controlled through a programmable light from a laser diode. This novel water analysis platform required only 500 mu L of sample (100 mu L for each nutrient) and 10-30 mu L of reagents for colorimetric detection. In addition, the fully automated parallel processes and efficient mixing in the rotating disc allowed for a significant reduction in total analysis time (similar to 7 min 40 s) and increased accuracy. Validation with a seawater certified reference material indicated that the platform accurately measured nutrient concentrations in water samples. In addition, we showed that the nutrients in the seawater collected from Chunsu Bay in Korea measured by the proposed lab-on-a-disc and by a commercialized autoanalyzer are comparable.
URI
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DOI
http://dx.doi.org/10.1021/ac3036734
ISSN
0003-2700
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