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Author

Park, Jung-Hoon
Bio-Optics Lab
Research Interests
  • Biomedical optics, wavefront shaping, In-vivo deep tissue imaging

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High-resolution in vivo imaging of mouse brain through the intact skull

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Title
High-resolution in vivo imaging of mouse brain through the intact skull
Author
Park, Jung-HoonSun, WeiCui, Meng
Keywords
Adaptive optics; Immunology; Neuroimaging; Nonlinear microscopy; Wavefront shaping
Issue Date
201507
Publisher
NATL ACAD SCIENCES
Citation
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v.112, no.30, pp.9236 - 9241
Abstract
Multiphoton microscopy is the current method of choice for in vivo deep-tissue imaging. The long laser wavelength suffers less scattering, and the 3D-confined excitation permits the use of scattered signal light. However, the imaging depth is still limited because of the complex refractive index distribution of biological tissue, which scrambles the incident light and destroys the optical focus needed for high resolution imaging. Here, we demonstrate a wavefront-shaping scheme that allows clear imaging through extremely turbid biological tissue, such as the skull, over an extended corrected field of view (FOV). The complex wavefront correction is obtained and directly conjugated to the turbid layer in a noninvasive manner. Using this technique, we demonstrate in vivo submicron-resolution imaging of neural dendrites and microglia dynamics through the intact skulls of adult mice. This is the first observation, to our knowledge, of dynamic morphological changes of microglia through the intact skull, allowing truly noninvasive studies of microglial immune activities free from external perturbations.
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DOI
http://dx.doi.org/10.1073/pnas.1505939112
ISSN
0027-8424
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