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Lim, Chunghun (임정훈)

Department
생명과학과
Website
https://sites.google.com/view/thelimlab/
Lab
Neurogenetics & Ribonomics Lab. (Neurogenetics & Ribonomics 연구실)
Research Keywords
전사 후 유전자 발현, 퇴행성 뇌질환, 행동신경유전, 분자신경생물, Post-transcriptional Gene Expression, Neurodegeneration, Behavioral Genetics, Molecular Neurobiology
Research Interests
# Current Research1. Decoding Non-canonical TranslationGene expression is central to all facets of physiology and its mechanistic basis had long been studied in the context of classical paradigm of transcription and translation. However, recent advances in RNA biology have revealed the robustness of “non-canonical” translation of which initiation occurs at non-AUG codons that associate with repetitive sequences, or at near-cognate start codons (i.e., AUG-like codon sequences), challenging a very fundamental principle of translation initiation. What remain to be addressed would be: 1) how translation factors and ribosomal machinery could “read” the code on mRNA molecules to initiate different types of non-canonical translation, and 2) why animals have evolved these unusual translation mechanisms on top of the canonical one. We employ molecular, genetic, genomics, and biochemical strategies (e.g., CRISPR/Cas9, RNA-seq, and mass-spec) to answer these fundamental questions and discover novel molecular principles underlying non-canonical translation in human cell cultures. Given that non-canonical translation has been shown to contribute to the expression of viral proteins or pathogenic cellular proteins relevant to neurological disorders, our findings should have clear, clinical implication in the development of new anti-viral drugs and better treatment of neurodegeneration.
2. Molecular Mechanisms Underlying Neurodegenerative DiseasesEmerging evidence indicates that post-transcriptional gene expression (i.e., molecular mechanisms that regulate gene expression after mRNAs are transcribed from DNA template) plays crucial roles in supporting neural development, function and physiology. Consistently, genetic mutations in RNA-binding proteins and regulatory RNAs have been shown to closely associate with neurological disorders in human. We previously demonstrated that an RNA-binding protein ATAXIN-2 forms two functionally distinct protein complexes with its associating factors LSM12 and ME31B/DDX6, respectively, to sustain circadian locomotor behaviors (e.g., daily sleep-wake cycles). We now expand our working model of the ATAXIN-2 function in neurodegenerative disease models of human induced pluripotent stem cell (iPSC) cultures to elucidate why genetic mutations in human ATAXIN-2 cause neurodegenerative diseases such as spinocerebellar ataxia type 2 (SCA2) and amyotrophic lateral sclerosis (ALS; also known as Lou Gehrig’s disease). Our studies will help develop novel therapeutic strategy to efficiently treat those neurological disorders relevant to ATAXIN-2.
3. Neural and Genetic Bases of Sleep Behaviors and Sleep-relevant PhysiologySleep is essential physiology that is well-conserved among animal species. However, molecular and neural mechanisms underlying sleep behaviors still remain elusive. We exploit Drosophila as our model system to understand 1) how our genes and neurons are functionally organized into sleep-regulatory pathways to support sleep homeostasis; 2) how the sleep-regulatory pathways intimately interact with the environment to adaptively adjust sleep behaviors, thereby manifesting sleep plasticity; and 3) how a lack of sleep could dominantly affect higher-order brain function and metabolism. Our studies will provide the neuro-genetic landscape of sleep regulation in Drosophila, hinting the fundamental principles of how human brain reciprocally controls sleep along with other sleep-relevant physiology.
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Issue DateTitleAuthor(s)TypeViewAltmetrics
2007-07Functional role of CREB-binding protein in the circadian clock system of Drosophila melanogasterLim, Chunghun; Lee, Jongbin; Choi, Changtaek, et alARTICLE594 Functional role of CREB-binding protein in the circadian clock system of Drosophila melanogaster
2007-06clockwork orange encodes a transcriptional repressor important for circadian-clock amplitude in DrosophilaLim, Chunghun; Chung, Brian Y.; Pitman, Jena L., et alARTICLE529 clockwork orange encodes a transcriptional repressor important for circadian-clock amplitude in Drosophila
2004-08Inhibition of nuclear factor kappa B activity by viral interferon regulatory factor 3 of Kaposi's sarcoma-associated herpesvirusSeo, Taegun; Park, Junsoo; Lim, Chunghun, et alARTICLE525 Inhibition of nuclear factor kappa B activity by viral interferon regulatory factor 3 of Kaposi's sarcoma-associated herpesvirus
2004-07Mitotic chromosome-binding activity of latency-associated nuclear antigen 1 is required for DNA replication from terminal repeat sequence of Kaposi's sarcoma-associated herpesvirusLim, Chunghun; Choi, Changtaek; Choe, JoonhoARTICLE565 Mitotic chromosome-binding activity of latency-associated nuclear antigen 1 is required for DNA replication from terminal repeat sequence of Kaposi's sarcoma-associated herpesvirus
2004-04Identification of a virus trans-acting regulatory element on the latent DNA replication of Kaposi's sarcoma-associated herpesvirusLim, Chunghun; Seo, Taegun; Jung, Jun, et alARTICLE477 Identification of a virus trans-acting regulatory element on the latent DNA replication of Kaposi's sarcoma-associated herpesvirus
2003-02Latency-associated nuclear antigen of Kaposi's sarcoma-associated herpesvirus functionally interacts with heterochromatin protein 1Lim, Chunghun; Lee, Daeyoup; Seo, Taegun, et alARTICLE555 Latency-associated nuclear antigen of Kaposi's sarcoma-associated herpesvirus functionally interacts with heterochromatin protein 1
2003-02The GIT family of proteins forms multimers and associates with the presynaptic cytomatrix protein PiccoloKim, Seho; Ko, Jaewon; Shin, Hyewon, et alARTICLE498 The GIT family of proteins forms multimers and associates with the presynaptic cytomatrix protein Piccolo
2002-12The viral oncogene human papillomavirus E7 deregulates transcriptional silencing by Brm-related gene 1 via molecular interactionsLee, Daeyoup; Lim, Chunghun; Seo, Taegun, et alARTICLE526 The viral oncogene human papillomavirus E7 deregulates transcriptional silencing by Brm-related gene 1 via molecular interactions
2002-10Functional dissection of latency-associated nuclear antigen 1 of Kaposi's sarcoma-associated herpesvirus involved in latent DNA replication and transcription of terminal repeats of the viral genomeLim, Chunghun; Sohn, Hekwang; Lee, Daeyoup, et alARTICLE534 Functional dissection of latency-associated nuclear antigen 1 of Kaposi's sarcoma-associated herpesvirus involved in latent DNA replication and transcription of terminal repeats of the viral genome
2002-02Kaposi's sarcoma-associated herpesvirus open reading frame 50 stimulates the transcriptional activity of STAT3Gwack, Yousang; Hwang, Seungmin; Lim, Chunghun, et alARTICLE591 Kaposi's sarcoma-associated herpesvirus open reading frame 50 stimulates the transcriptional activity of STAT3
2001-10The Kaposi's sarcoma-associated herpesvirus K8 protein interacts with CREB-binding protein (CBP) and represses CBP-mediated transcriptionHwang, SEUNGMIN; Gwack, YOUSANG; Byun, HYEWON, et alARTICLE499 The Kaposi's sarcoma-associated herpesvirus K8 protein interacts with CREB-binding protein (CBP) and represses CBP-mediated transcription
2001-08The transcriptional activity of cAMP response element-binding protein-binding protein is modulated by the latency associated nuclear antigen of Kaposi's sarcoma-associated herpesvirusLim, Chunghun; Gwack, Yousang; Hwang, Seungmin, et alARTICLE492 The transcriptional activity of cAMP response element-binding protein-binding protein is modulated by the latency associated nuclear antigen of Kaposi's sarcoma-associated herpesvirus
2001-07Kaposi's sarcoma-associated herpesvirus open reading frame 50 represses p53-induced transcriptional activity and apoptosisGwack, YOUSANG; Hwang, SEUNGMIN; Byun, HYEWON, et alARTICLE582 Kaposi's sarcoma-associated herpesvirus open reading frame 50 represses p53-induced transcriptional activity and apoptosis
2001-02CREB-binding protein and histone deacetylase regulate the transcriptional activity of Kaposi's sarcoma-associated herpesvirus open reading frame 50Gwack, YOUSANG; Byun, HYEWON; Hwang, SEUNGMIN, et alARTICLE511 CREB-binding protein and histone deacetylase regulate the transcriptional activity of Kaposi's sarcoma-associated herpesvirus open reading frame 50
2000-11Latency-associated nuclear antigen of Kaposi's sarcoma-associated herpesvirus (human herpesvirus-8) binds ATF4/CREB2 and inhibits its transcriptional activation activityLim, Chunghun; Sohn, Hekwang; Gwack, Yousang, et alARTICLE515

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