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Lim, Chunghun (임정훈)

Department
Department of Biological Sciences(생명과학과)
Website
https://sites.google.com/view/thelimlab/
Lab
Neurogenetics & Ribonomics Lab. (Neurogenetics & Ribonomics 연구실)
Research Keywords
분자신경생물, 단백질 번역, 퇴행성 뇌질환, 행동신경유전, Molecular Neurobiology, Ribosome Biology, Translation, Neurodegeneration, Behavioral Genetics
Research Interests
# Current Research
1. Decoding Non-canonical TranslationGene expression is central to all facets of physiology and its mechanistic basis had long been studied in the context of classical paradigm of transcription and translation. However, recent advances in RNA biology have revealed the robustness of “non-canonical” translation of which initiation occurs at non-AUG codons that associate with repetitive sequences, or at near-cognate start codons (i.e., AUG-like codon sequences), challenging a very fundamental principle of translation initiation. What remain to be addressed would be: 1) how translation factors and ribosomal machinery could “read” the code on mRNA molecules to initiate different types of non-canonical translation, and 2) why animals have evolved these unusual translation mechanisms on top of the canonical one. We employ molecular, genetic, genomics, and biochemical strategies (e.g., CRISPR/Cas9, RNA-seq, and mass-spec) to answer these fundamental questions and discover novel molecular principles underlying non-canonical translation in human cell cultures. Given that non-canonical translation has been shown to contribute to the expression of viral proteins or pathogenic cellular proteins relevant to neurological disorders, our findings should have clear, clinical implication in the development of new anti-viral drugs and better treatment of neurodegeneration.
2. Molecular Mechanisms Underlying Neurodegenerative DiseasesEmerging evidence indicates that post-transcriptional gene expression (i.e., molecular mechanisms that regulate gene expression after mRNAs are transcribed from DNA template) plays crucial roles in supporting neural development, function, and physiology. Consistently, genetic mutations in RNA-binding proteins and regulatory RNAs have been shown to closely associate with neurological disorders in human. We previously demonstrated that an RNA-binding protein ATAXIN-2 forms two functionally distinct protein complexes with its associating factors LSM12 and ME31B/DDX6, respectively, to sustain circadian locomotor behaviors (e.g., daily sleep-wake cycles). We now expand our working model to degeneration in iPSC(induced pluripotent stem cells)-derived motor neurons from ALS patients (amyotrophic lateral sclerosis; also known as Lou Gehrig’s disease) and transgenic Drosophila neurons to elucidate how genetic mutations in RNA-binding proteins and regulatory RNAs cause neurodegenerative diseases such as ALS and frontotemporal dementia (FTD). Our studies will help develop novel therapeutic strategy to efficiently treat those RNA-relevant neurological disorders.
3. Neural and Genetic Bases of Sleep Behaviors and Sleep-relevant PhysiologySleep is essential physiology that is well-conserved among animal species. However, molecular and neural mechanisms underlying sleep behaviors still remain elusive. We exploit Drosophila as our model system to understand 1) how our genes and neurons are functionally organized into sleep-regulatory pathways to support sleep homeostasis; 2) how the sleep-regulatory pathways intimately interact with the environment to adaptively adjust sleep behaviors, thereby manifesting sleep plasticity; and 3) how a lack of sleep could dominantly affect higher-order brain function and metabolism. Our studies will provide the neuro-genetic landscape of sleep regulation in Drosophila, hinting the fundamental principles of how human brain reciprocally controls sleep along with other sleep-relevant physiology.
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Issue DateTitleAuthor(s)TypeViewAltmetrics
2020-10A sleep-like state in Hydra unravels conserved sleep mechanisms during the evolutionary development of the central nervous systemKanaya, Hiroyuki J.; Park, Sungeon; Kim, Ji-hyung, et alARTICLE44 A sleep-like state in Hydra unravels conserved sleep mechanisms during the evolutionary development of the central nervous system
2020-04The voltage-gated potassium channel Shaker promotes sleep via thermosensitive GABA transmissionKim, Ji-hyung; Ki, Yoonhee; Lee, Hoyeon, et alARTICLE141 The voltage-gated potassium channel Shaker promotes sleep via thermosensitive GABA transmission
2020-02hnRNP K Supports High-Amplitude D Site-Binding Protein mRNA (Dbp mRNA) Oscillation To Sustain Circadian RhythmsKwon, Paul Kwangho; Lee, Kyung-Ha; Kim, Ji-hyung, et alARTICLE99 hnRNP K Supports High-Amplitude D Site-Binding Protein mRNA (Dbp mRNA) Oscillation To Sustain Circadian Rhythms
2019-10Ataxin2 functions via CrebA to mediate Huntingtin toxicity in circadian clock neuronsXu, Fangke; Kula-Eversole, Elzbieta; Iwanaszko, Marta, et alARTICLE221 Ataxin2 functions via CrebA to mediate Huntingtin toxicity in circadian clock neurons
2019-07Sleep-promoting effects of threonine link amino acid metabolism in Drosophila neuron to GABAergic control of sleep driveKi, Yoonhee; Lim, ChunghunARTICLE219 Sleep-promoting effects of threonine link amino acid metabolism in Drosophila neuron to GABAergic control of sleep drive
2019-04Drosophila CrebB is a Substrate of the Nonsense-Mediated mRNA Decay Pathway that Sustains Circadian BehaviorsRi, Hwajung; Lee, Jongbin; Sonn, Jun Young, et alARTICLE149 Drosophila CrebB is a Substrate of the Nonsense-Mediated mRNA Decay Pathway that Sustains Circadian Behaviors
2018-11Ataxin-2: A versatile posttranscriptional regulator and its implication in neural functionLee, Jongbo; Kim, Minjong; Itoh, Taich Q, et alARTICLE429 Ataxin-2: A versatile posttranscriptional regulator and its implication in neural function
2018-07Serine metabolism in the brain regulates starvation-induced sleep suppression in Drosophila melanogasterSonn, Jun Young; Lee, Jongbin; Sung, Min Kyung, et alARTICLE412 Serine metabolism in the brain regulates starvation-induced sleep suppression in Drosophila melanogaster
2018-07High-Amplitude Circadian Rhythms in Drosophila Driven by Calcineurin-Mediated Post-translational Control of sarahKweon, Sin Ho; Lee, Jongbin; Lim, Chunghun, et alARTICLE421 High-Amplitude Circadian Rhythms in Drosophila Driven by Calcineurin-Mediated Post-translational Control of sarah
2017-09Rogdi Defines GABAergic Control of a Wake-promoting Dopaminergic Pathway to Sustain Sleep in DrosophilaKim, Minjong; Jang, Donghoon; Yoo, Eunseok, et alARTICLE553 Rogdi Defines GABAergic Control of a Wake-promoting Dopaminergic Pathway to Sustain Sleep in Drosophila
2017-06The crystal structure of human Rogdi provides insight into the causes of Kohlschutter-Tonz SyndromeLee, Hakbong; Jeong, Hanbin; Choe, Joonho, et alARTICLE562 The crystal structure of human Rogdi provides insight into the causes of Kohlschutter-Tonz Syndrome
2017-04LSM12 and ME31B/DDX6 Define Distinct Modes of Posttranscriptional Regulation by ATAXIN-2 Protein Complex in Drosophila Circadian Pacemaker NeuronsLee, Jongbo; Yoo, Eunseok; Lee, Hoyeon, et alARTICLE605 LSM12 and ME31B/DDX6 Define Distinct Modes of Posttranscriptional Regulation by ATAXIN-2 Protein Complex in Drosophila Circadian Pacemaker Neurons
2016-08CRTC Potentiates Light-independent timeless Transcription to Sustain Circadian Rhythms in DrosophilaKim, Minkyung; Lee, Hoyeon; Hur, Jin-Hoe, et alARTICLE576 CRTC Potentiates Light-independent timeless Transcription to Sustain Circadian Rhythms in Drosophila
2015-10Warming Up Your Tick-Tock: Temperature-Dependent Regulation of Circadian ClocksKi, Yoonhee; Ri, Hwajung; Lee, Hoyeon, et alARTICLE770 Warming Up Your Tick-Tock: Temperature-Dependent Regulation of Circadian Clocks
2014-04SIFamide and SIFamide receptor define a novel neuropeptide signaling to promote sleep in DrosophilaPark, Sangjin; Sonn, Jun Young; Oh, Yangkyun, et alARTICLE721 SIFamide and SIFamide receptor define a novel neuropeptide signaling to promote sleep in Drosophila
2013-11Emerging roles for post-transcriptional regulation in circadian clocksLim, Chunghun; Allada, RaviARTICLE677 Emerging roles for post-transcriptional regulation in circadian clocks
2013-05ATAXIN-2 activates PERIOD translation to sustain circadian rhythms in DrosophilaLim, Chunghun; Allada, RaviARTICLE714 ATAXIN-2 activates PERIOD translation to sustain circadian rhythms in Drosophila
2011-02The novel gene twenty-four defines a critical translational step in the Drosophila clockLim, Chunghun; Lee, Jongbin; Choi, Changtaek, et alARTICLE780 The novel gene twenty-four defines a critical translational step in the Drosophila clock
2010-04DNA-PK/Ku complex binds to latency-associated nuclear antigen and negatively regulates Kaposi's sarcoma-associated herpesvirus latent replicationCha, Seho; Lim, Chunghun; Lee, Jae Young, et alARTICLE551 DNA-PK/Ku complex binds to latency-associated nuclear antigen and negatively regulates Kaposi's sarcoma-associated herpesvirus latent replication
2009-10The DOUBLETIME protein kinase regulates phosphorylation of the Drosophila PDP1 epsilonChoi, Changtaek; Lee, Jongbin; Lim, Chunghun, et alARTICLE569 The DOUBLETIME protein kinase regulates phosphorylation of the Drosophila PDP1 epsilon

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