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Yoo, Jung-Woo
Nano Spin Transport Lab.
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dc.citation.endPage 270 -
dc.citation.number 1-2 -
dc.citation.startPage 263 -
dc.citation.title INTERNATIONAL JOURNAL OF PHARMACEUTICS -
dc.citation.volume 358 -
dc.contributor.author Pan, Xiaogang -
dc.contributor.author Guan, Jingjiao -
dc.contributor.author Yoo, Jung-Woo -
dc.contributor.author Epstein, Arthur J. -
dc.contributor.author Lee, L. James -
dc.contributor.author Lee, Robert J. -
dc.date.accessioned 2023-12-22T08:39:21Z -
dc.date.available 2023-12-22T08:39:21Z -
dc.date.created 2015-01-05 -
dc.date.issued 2008-06 -
dc.description.abstract Cationic lipid-coated magnetic nanoparticles (MPs) associated with transferrin were evaluated as gene transfer vectors in the presence of a static magnetic field. MPs were prepared by chemical precipitation and were surface-coated with cationic lipids, composed of DDAB/soy PC (60:40mole/mole). These cationic MPs were then combined with polyethylenimine (PEI) condensed plasmid DNA, followed by transferrin. The resulting magnetic electrostatic complexes retained relatively compact particle size and showed complete DNA condensation. Their transfection activity in the presence of a static magnetic field was evaluated by luciferase and green fluorescent protein (GFP) reporter genes. The magnetic complexes exhibited up to 300-fold higher transfection activity compared to commonly used cationic liposomes or cationic polymer complexes, based on luciferase assay. The enhancement in transfection activity was maximized when the cells were exposed to the vectors for a relatively short period of time (15 min), or were treated in media containing 10% serum. Incorporation of transferrin further improved transfection efficiency of the cationic MPs. However, when cells were incubated for 4 h in serum-free media, magnetic and non-magnetic vectors showed similar transfection efficiencies. in conclusion, transferrin-associated cationic MPs are excellent gene transfer vectors that can mediate very rapid and efficient gene transfer in vitro in the presence of a magnetic field. -
dc.identifier.bibliographicCitation INTERNATIONAL JOURNAL OF PHARMACEUTICS, v.358, no.1-2, pp.263 - 270 -
dc.identifier.doi 10.1016/j.ijpharm.2008.02.020 -
dc.identifier.issn 0378-5173 -
dc.identifier.scopusid 2-s2.0-44449133523 -
dc.identifier.uri https://scholarworks.unist.ac.kr/handle/201301/9831 -
dc.identifier.url http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=44449133523 -
dc.identifier.wosid 000257577400036 -
dc.language 영어 -
dc.publisher ELSEVIER SCIENCE BV -
dc.title Cationic lipid-coated magnetic nanoparticles associated with transferrin for gene delivery -
dc.type Article -
dc.description.journalRegisteredClass scopus -
dc.subject.keywordAuthor gene delivery -
dc.subject.keywordAuthor magnetic nanoparticles -
dc.subject.keywordAuthor transferrin -
dc.subject.keywordAuthor cationic lipid -
dc.subject.keywordAuthor magnetofection -
dc.subject.keywordPlus IN-VITRO -
dc.subject.keywordPlus DNA -
dc.subject.keywordPlus CELLS -
dc.subject.keywordPlus TRANSFECTION -
dc.subject.keywordPlus MAGNETOFECTION -
dc.subject.keywordPlus COMPLEXES -
dc.subject.keywordPlus EXPRESSION -
dc.subject.keywordPlus THERAPY -
dc.subject.keywordPlus VIVO -
dc.subject.keywordPlus POLYETHYLENIMINE -

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