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Yoo, Jung-Woo
Functional Hybrid Materials and Devices Lab(FHMDL)
Research Interests
  • Transport properties, spintronics, organic/molucular magnet, electronic, and spintronics,
  • 2DEG, energy harvesting device, atomic/molecular layer deposition

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Cationic lipid-coated magnetic nanoparticles associated with transferrin for gene delivery

Cited 48 times inthomson ciCited 52 times inthomson ci
Title
Cationic lipid-coated magnetic nanoparticles associated with transferrin for gene delivery
Author
Pan, XiaogangGuan, JingjiaoYoo, Jung-WooEpstein, Arthur J.Lee, L. JamesLee, Robert J.
Keywords
Cationic lipid; Gene delivery; Magnetic nanoparticles; Magnetofection; Transferrin
Issue Date
2008-06
Publisher
ELSEVIER SCIENCE BV
Citation
INTERNATIONAL JOURNAL OF PHARMACEUTICS, v.358, no.1-2, pp.263 - 270
Abstract
Cationic lipid-coated magnetic nanoparticles (MPs) associated with transferrin were evaluated as gene transfer vectors in the presence of a static magnetic field. MPs were prepared by chemical precipitation and were surface-coated with cationic lipids, composed of DDAB/soy PC (60:40mole/mole). These cationic MPs were then combined with polyethylenimine (PEI) condensed plasmid DNA, followed by transferrin. The resulting magnetic electrostatic complexes retained relatively compact particle size and showed complete DNA condensation. Their transfection activity in the presence of a static magnetic field was evaluated by luciferase and green fluorescent protein (GFP) reporter genes. The magnetic complexes exhibited up to 300-fold higher transfection activity compared to commonly used cationic liposomes or cationic polymer complexes, based on luciferase assay. The enhancement in transfection activity was maximized when the cells were exposed to the vectors for a relatively short period of time (15 min), or were treated in media containing 10% serum. Incorporation of transferrin further improved transfection efficiency of the cationic MPs. However, when cells were incubated for 4 h in serum-free media, magnetic and non-magnetic vectors showed similar transfection efficiencies. in conclusion, transferrin-associated cationic MPs are excellent gene transfer vectors that can mediate very rapid and efficient gene transfer in vitro in the presence of a magnetic field.
URI
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DOI
10.1016/j.ijpharm.2008.02.020
ISSN
0378-5173
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MSE_Journal Papers
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