BROWSE

Related Researcher

Author's Photo

Lee, Changwook
Structural Biology of Cellular Biochemistry Lab
Research Interests
  • Cell biology, structural biology, protein biochemistry, membrane biology, cancer biology

ITEM VIEW & DOWNLOAD

Crystallization and preliminary X-ray diffraction analysis of the Sel1-like repeats of SEL1L

Cited 0 times inthomson ciCited 0 times inthomson ci
Title
Crystallization and preliminary X-ray diffraction analysis of the Sel1-like repeats of SEL1L
Author
Jeong, HanbinLee, HakbongLee, Changwook
Issue Date
2014-12
Publisher
WILEY-BLACKWELL
Citation
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS, v.70, no.12, pp.1624 - 1627
Abstract
Terminally misfolded or unassembled proteins are selectively recognized and cleared by the ER-associated degradation (ERAD) pathway. Suppressor/enhancer of lin-12-like (SEL1L), a component of the dislocation machinery containing the E3 ubiquitin ligase Hrd1, plays an important role in selecting and transporting ERAD substrates for degradation in the endoplasmic reticulum. In this study, the purification, crystallization and preliminary X-ray diffraction analysis of recombinant mouse SEL1L (residues 348-533) are reported. The crystals were obtained by the hanging-drop vapour-diffusion method at pH 8.5 and 277 K using 30% 2-propanol as a precipitant. Optimized crystals diffracted to 3.3 angstrom resolution at a synchrotron-radiation source. Preliminary X-ray diffraction analysis revealed that the crystals belonged to space group P2(1) and contained four molecules per asymmetric unit, with a solvent content of 44%.
URI
https://scholarworks.unist.ac.kr/handle/201301/9786
URL
http://scripts.iucr.org/cgi-bin/paper?S2053230X14023115
DOI
10.1107/S2053230X14023115
ISSN
2053-230X
Appears in Collections:
BIO_Journal Papers
Files in This Item:
There are no files associated with this item.

find_unist can give you direct access to the published full text of this article. (UNISTARs only)

Show full item record

qrcode

  • mendeley

    citeulike

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

MENU