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Bhak, Jong
KOrean GenomIcs Center(KOGIC)
Research Interests
  • Geromics, genomics, bioinformatics, protein Engineering, OMICS


De novo transcriptome sequencing of Momordica cochinchinensis to identify genes involved in the carotenoid biosynthesis

DC Field Value Language Hyun, Tae Kyung ko Rim, Yeonggil ko Jang, Hui-Jeong ko Kim, Cheol Hong ko Park, Jongsun ko Kumar, Ritesh ko Lee, Sunghoon ko Kim, Byung Chul ko Bhak, Jong Hwa ko Binh Nguyen-Quoc ko Kim, Seon-Won ko Lee, Sang Yeol ko Kim, Jae-Yean ko 2014-12-26T00:13:34Z - 2014-12-24 ko 2012-07 -
dc.identifier.citation PLANT MOLECULAR BIOLOGY, v.79, no.4-5, pp.413 - 427 ko
dc.identifier.issn 0167-4412 ko
dc.identifier.uri -
dc.identifier.uri ko
dc.description.abstract The ripe fruit of Momordica cochinchinensis Spreng, known as gac, is featured by very high carotenoid content. Although this plant might be a good resource for carotenoid metabolic engineering, so far, the genes involved in the carotenoid metabolic pathways in gac were unidentified due to lack of genomic information in the public database. In order to expedite the process of gene discovery, we have undertaken Illumina deep sequencing of mRNA prepared from aril of gac fruit. From 51,446,670 high-quality reads, we obtained 81,404 assembled unigenes with average length of 388 base pairs. At the protein level, gac aril transcripts showed about 81.5 % similarity with cucumber proteomes. In addition 17,104 unigenes have been assigned to specific metabolic pathways in Kyoto Encyclopedia of Genes and Genomes, and all of known enzymes involved in terpenoid backbones biosynthetic and carotenoid biosynthetic pathways were also identified in our library. To analyze the relationship between putative carotenoid biosynthesis genes and alteration of carotenoid content during fruit ripening, digital gene expression analysis was performed on three different ripening stages of aril. This study has revealed putative phytoene synthase, 15-cis-phytone desaturase, zeta-carotene desaturase, carotenoid isomerase and lycopene epsilon cyclase might be key factors for controlling carotenoid contents during aril ripening. Taken together, this study has also made availability of a large gene database. This unique information for gac gene discovery would be helpful to facilitate functional studies for improving carotenoid quantities. ko
dc.description.statementofresponsibility close -
dc.language ENG ko
dc.publisher SPRINGER ko
dc.subject Carotenoid ko
dc.subject Digital gene expression ko
dc.subject Momordica cochinchinensis Spreng ko
dc.subject Next generation sequencing ko
dc.subject Transcriptome ko
dc.title De novo transcriptome sequencing of Momordica cochinchinensis to identify genes involved in the carotenoid biosynthesis ko
dc.type ARTICLE ko
dc.identifier.scopusid 2-s2.0-84862000006 ko
dc.identifier.wosid 000304913200007 ko
dc.type.rims ART ko
dc.description.wostc 13 *
dc.description.scopustc 9 * 2015-05-06 * 2014-12-24 *
dc.identifier.doi 10.1007/s11103-012-9919-9 ko
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