Multiphasic Sensory Alginate Particle Having Polydiacetylene Liposome for Selective and More Sensitive Multitargeting Detection

Abstract

A novel fabrication method of monophasic, biphasic, and triphasic alginate microparticles having sensory polydiacetylene (FDA) liposomes has been developed to achieve selective and more sensitive multitargeting detection in solution. In this alginate microparticle based detection system, the sensory PDA liposomes are concentrated in the particles rather than being diluted in a solution, which is the case of a conventional solution based detection system, providing superior sensitivity and stability. The biphasic nature of the alginate microparticles was realized by coinjecting two different PDA liposome/alginate mixture solutions into a CaCl2 solution using a simple combined needle injection system. The size and the constituent of the Janus particles and the extended triphasic particles could be independently manipulated by controlling a centrifugal force and formulating the composition of the PDA liposome solutions, respectively. The multitargeting capability of such mutiphasic alginate particles was demonstrated by fluorescence microscopy The presented particle-based detection system has a great potential to be combined with a microfluidic device for the development of advanced biosensors having a high throughput screening capability.