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Lee, Changsoo
Applied Biotechnology Lab for Environment
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dc.citation.endPage 327 -
dc.citation.startPage 321 -
dc.citation.title BIORESOURCE TECHNOLOGY -
dc.citation.volume 174 -
dc.contributor.author Kim, Jaai -
dc.contributor.author Lee, Changsoo -
dc.date.accessioned 2023-12-22T01:49:38Z -
dc.date.available 2023-12-22T01:49:38Z -
dc.date.created 2014-12-02 -
dc.date.issued 2014-12 -
dc.description.abstract Characterizing microbial community structure using molecular techniques is becoming a popular approach in studies of waste/wastewater treatment processes. A rapid and robust tool to analyze microbial communities is required for efficient process monitoring and control. In this study, a new community fingerprinting method based on high-resolution melting (HRM) analysis was developed and applied to compare methanogenic community structures of five different anaerobic digesters. The new method produced robust community clustering and ordination results comparable to the results from the commonly used denaturing gradient gel electrophoresis (DGGE) performed in parallel. This method transforms melting peak plots (MPs) of community DNA samples generated by HRM analysis to molecular fingerprints and estimates the relationships between the communities based on the fingerprints. The MP-based fingerprinting would provide a good alternative to monitor variations in microbial community structure especially when handling large sample numbers due to its high-throughput capacity and short analysis time. -
dc.identifier.bibliographicCitation BIORESOURCE TECHNOLOGY, v.174, pp.321 - 327 -
dc.identifier.doi 10.1016/j.biortech.2014.10.037 -
dc.identifier.issn 0960-8524 -
dc.identifier.scopusid 2-s2.0-84910643490 -
dc.identifier.uri https://scholarworks.unist.ac.kr/handle/201301/9307 -
dc.identifier.url http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84910643490 -
dc.identifier.wosid 000345014900042 -
dc.language 영어 -
dc.publisher ELSEVIER SCI LTD -
dc.title Rapid fingerprinting of methanogenic communities by high-resolution melting analysis -
dc.type Article -
dc.relation.journalWebOfScienceCategory Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels -
dc.relation.journalResearchArea Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -
dc.subject.keywordAuthor Denaturing gradient gel electrophoresis -
dc.subject.keywordAuthor High-resolution melting analysis -
dc.subject.keywordAuthor Methanogenic community -
dc.subject.keywordAuthor Microbial community structure -
dc.subject.keywordAuthor Molecular fingerprinting -

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