BROWSE

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Madou, Mark
BIO-MEMS Lab
Research Interests
  • Medical Diagnostics
  • Micro-Battery Development
  • Drug Delivery Systems

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Centrifugal microfluidic platform for rapid PCR amplification using integrated thermoelectric heating and ice-valving

DC Field Value Language
dc.contributor.author Amasia, Mary ko
dc.contributor.author Cozzens, Meghan ko
dc.contributor.author Madou, Mark ko
dc.date.available 2014-11-25T00:13:18Z -
dc.date.created 2014-11-21 ko
dc.date.issued 2012-01 -
dc.identifier.citation SENSORS AND ACTUATORS B-CHEMICAL, v.161, no.1, pp.1191 - 1197 ko
dc.identifier.issn 0925-4005 ko
dc.identifier.uri https://scholarworks.unist.ac.kr/handle/201301/9202 -
dc.identifier.uri http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84856221585 ko
dc.description.abstract We present a novel centrifugal microfluidic system for the rapid amplification of a Bacillus anthracis gene, with integrated pumping and thermoelectric-based heating and valving. Polymerase chain reaction is a powerful method for rapid and accurate diagnosis of infectious diseases and bio-warfare agents. The fully integrated system and disposable disc developed here automates the fluidic pumping, valving, and rapid thermocycling required for polymerase chain reaction. We demonstrate the first utilization of ice-valving in an integrated centrifugal microfluidic system as a method for sealing the thermocycling chamber to reduce fluid loss due to evaporation. The automated centrifugal system is validated via amplification of a B. anthracis gene. Fluidic thermal profiles are monitored and optimized to minimize non-specific amplification, reducing the total amplification time from 110 min down to 53 min. ko
dc.description.statementofresponsibility close -
dc.language ENG ko
dc.publisher ELSEVIER SCIENCE SA ko
dc.subject Centrifugal microfluidics ko
dc.subject Ice-valving ko
dc.subject PCR amplification ko
dc.title Centrifugal microfluidic platform for rapid PCR amplification using integrated thermoelectric heating and ice-valving ko
dc.type ARTICLE ko
dc.identifier.scopusid 2-s2.0-84856221585 ko
dc.identifier.wosid 000301549400163 ko
dc.type.rims ART ko
dc.description.wostc 19 *
dc.description.scopustc 15 *
dc.date.tcdate 2015-05-06 *
dc.date.scptcdate 2014-11-21 *
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