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Kim, Chae Un
High Pressure X-ray Science Lab (HiPreX)
Research Interests
  • X-ray Science, biophysical science, biomedical science

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Cryogenic x-ray diffraction microscopy utilizing high-pressure cryopreservation

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Title
Cryogenic x-ray diffraction microscopy utilizing high-pressure cryopreservation
Author
Lima, EnjuChushkin, YuriyVan Der Linden, PeterKim, Chae UnZontone, FedericoCarpentier, PhilippeGruner, Sol M.Pernot, Petra
Issue Date
2014-10
Publisher
AMER PHYSICAL SOC
Citation
PHYSICAL REVIEW E, v.90, no.4, pp.042713
Abstract
We present cryo x-ray diffraction microscopy of high-pressure-cryofixed bacteria and report high-convergence imaging with multiple image reconstructions. Hydrated D. radiodurans cells were cryofixed at 200 MPa pressure into ∼10-μm-thick water layers and their unstained, hydrated cellular environments were imaged by phasing diffraction patterns, reaching sub-30-nm resolutions with hard x-rays. Comparisons were made with conventional ambient-pressure-cryofixed samples, with respect to both coherent small-angle x-ray scattering and the image reconstruction. The results show a correlation between the level of background ice signal and phasing convergence, suggesting that phasing difficulties with frozen-hydrated specimens may be caused by high-background ice scattering.
URI
https://scholarworks.unist.ac.kr/handle/201301/9144
URL
http://journals.aps.org/pre/abstract/10.1103/PhysRevE.90.042713
DOI
10.1103/PhysRevE.90.042713
ISSN
2470-0045
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PHY_Journal Papers
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