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Suh, Pann-Ghill
BioSignal Network Lab (BSN)
Research Interests
  • Signal transduction, cancer, metabolism, phospholipase C

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Targeted label-free quantitative analysis of secretory proteins from adipocytes in response to oxidative stress

Cited 10 times inthomson ciCited 11 times inthomson ci
Title
Targeted label-free quantitative analysis of secretory proteins from adipocytes in response to oxidative stress
Author
Choi, S.Kim, J.Yea, K.Suh, Pann-GhillKim, J.Ryu, S.H.
Issue Date
2010-06
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Citation
ANALYTICAL BIOCHEMISTRY, v.401, no.2, pp.196 - 202
Abstract
Adipocytes are well known to release regulation factors associated with metabolic disorders. In particular, increased oxidative stress in adipocytes contributes to dysregulation of adipokine production. In this study, we applied relative quantitative proteomic analysis based on label-free multiple reaction monitoring (MRM) to discover biological changes of adipokines under oxidative stress. Among a total of 194 identified proteins, 8 proteins were selected and quantified between control and hydrogen peroxide (H2O2)-treated groups by label-free MRM quantification. The secretion levels of matrix metalloproteinase-2 (MMP-2), stromal cell-derived factor-1 (SDF-1, CXCL12), resistin, and complement factor D (CFD, adipsin) decreased, whereas the secretion levels of tissue inhibitor of metalloproteinase-2 (TIMP-2) and aldolase A increased. Here we suggest that our study with label-free quantitative analysis will contribute to the efficient quantitative analysis of targeted proteins in complex mixtures and specifically to a better understanding of changes of adipokines under oxidative stress.
URI
https://scholarworks.unist.ac.kr/handle/201301/9003
URL
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=77952851155
DOI
10.1016/j.ab.2010.03.006
ISSN
0003-2697
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BIO_Journal Papers
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