Altermagnets exhibit characteristics akin to antiferromagnets, with spin-split anisotropic bands in momentum space. RuO2 has been considered as a prototype altermagnet; however, recent reports have questioned altermagnetic ground state in this material. In this Letter, we demonstrate spin-dependent tunneling magnetoresistance (TMR) in RuO2-based magnetic tunnel junctions, which suggests the spinsplitted anisotropic band structure of our RuO2 films. The observed TMR is contingent on the direction of the Neel vector of RuO2 and reverse its sign by the inversion of the Neel vector. These results reflect the altermagnetic nature of RuO2 and highlight its potential for spintronic applications, leveraging the combined strengths of ferromagnetic and antiferromagnetic systems.