Graft-versus-host disease of the CNS is mediated by TNF upregulation in microglia

Abstract

Acute graft-versus-host disease (GVHD) can affect the central nervous system (CNS). The role of microglia in CNS-GVHD remains undefined. In agreement with microglia activation, we found that profound morphological changes and MHC-II and CD80 upregulation occurred upon GVHD induction. RNA sequencing-based analysis of purified microglia obtained from mice with CNS-GVHD revealed TNF upregulation. Selective TNF gene deletion in microglia of Cx3crT(crE )Tn(fl/)(-) mice reduced MHC-II expression and decreased CNS T cell infiltrates and VCAM-1(+) endothelial cells. GVHD increased microglia TGF-beta-activated kinase-1 (TAK1) activation and NF-kappa B/p38 MARK signaling. Selective Tak1 deletion in microglia using Cx3crT(crE )Tn(fl/)(fl) mice resulted in reduced TNF production and microglial MHC-II and improved neurocognitive activity. Pharmacological TAK1 inhibition reduced TNF production and MHC-II expression by microglia, Th1 and Th17 T cell infiltrates, and VCAM-1(+) endothelial cells and improved neurocognitive activity, without blocking graft-versus-leukemia effects. Consistent with these findings in mice, we observed increased activation and TNF production of microglia in the CNS of GVHD patients. In summary, we prove a role for microglia in CNS-GVHD, identify the TAK1/TNF/MHC-II axis as a mediator of CNS-GVHD, and provide a TAK1 inhibitor-based approach against GVHD-induced neurotoxicity.