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Visualization of nitric oxide in living cells by a copper-based fluorescent probe

Author(s)
Lim, Mi HeeXu, DLippard, SJ
Issued Date
2006-07
DOI
10.1038/nchembio794
URI
https://scholarworks.unist.ac.kr/handle/201301/8647
Fulltext
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=33745323369
Citation
NATURE CHEMICAL BIOLOGY, v.2, no.7, pp.375 - 380
Abstract
Nitric oxide (NO) serves as a messenger for cellular signaling. To visualize NO in living cells, we synthesized a turn-on fluorescent probe for use in combination with microscopy. Unlike existing fluorescent sensors, the construct-a Cu(II) complex of a fluorescein modified with an appended metal-chelating ligand (FL)-directly and immediately images NO rather than a derivative reactive nitrogen species. Using spectroscopic and mass spectrometric methods, we established that the mechanism of the reaction responsible for the NO-induced fluorescence involves reduction of the complex to Cu(I) with release of the nitrosated ligand, which occurs irreversibly. We detected NO produced by both constitutive and inducible NO synthases (cNOS and iNOS, respectively) in live neurons and macrophages in a concentration- and time-dependent manner by using the Cu(II)-based imaging agent. Both the sensitivity to nanomolar concentrations of NO and the spatiotemporal information provided by this complex demonstrate its value for numerous biological applications.
Publisher
NATURE PUBLISHING GROUP
ISSN
1552-4450
Keyword
ESCHERICHIA-COLINOBEL LECTURENITROSATIONCOMPLEXESCATALYSISBIOLOGY

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