SUSTAINABLE RELEASE OF RETINOIC ACID ENABLES ENHANCED NEUROAL STEM CELL DIFFERENTIATION BY MIMICKING IN VIVO MATRIX USING BIODEGRADABLE POROUS SILICON

Abstract

We address the limitations of current methodologies for deriving motor neurons from human induced pluripotent stem cells (hiPSCs) by introducing a biodegradable porous silicon microparticles. The inherent challenge of replicating the diversity and maturity of native motor neurons is overcome by strategically utilizing a biodegradable porous silicon matrix to secure the sustained release of the critical morphogen, retinoic acid (RA). RA plays a pivotal role in spinal cord development, yet its short half-life in physiological environments has impeded efficient neural differentiation. Our methodology involves loading hydrophobic RA molecules into the porous silicon matrix, effectively transforming it into a reservoir for controlled and prolonged release of RA. Through a systematic exploration of the structural parameters of the porous matrix, including pore size, particle size, and RA-sealing chemistry, we achieve precise manipulation of RA concentration in culture conditions. The results demonstrate a continuous and secure release of RA, maintaining its activity over an extended period.