Evolutionary diversification of RFX family genes as master regulators of ciliogenesis.

Abstract

Evolu&onary diversifica&on of RFX family genes as master regulators of ciliogenesis Ha Eun Kim, Taejoon Kwon*, Tae Joo Park* School of Biological Sciences, Ulsan Na2onal Ins2tute of Science and Technology (UNIST) Taejoon Kwon : tkwon@unist.ac.kr , Tae Joo Park : tjpark01@unist.ac.kr Regulatory factor X-box binding(RFX) family have well conserved and specialized winged-helix DNA binding domain. Previous studies reported that RFX gene family broadly interact with cilia genes. RFX2 stabilizes the binding of Foxj1 to the mulG-cilia gene promoter, to specifically promotes mulG-ciliated cell differenGaGon. RFX3 regulates ciliogenesis in embryonic node, pancreaGc endocrine cells and moGle cilia in brain. Also, RFX7 regulates neuronal ciliogenesis by regulaGng the expression level of RFX4. In situ hybridizaGon and Gssue expression data of Xenopus embryos revealed that some RFX genes are expressed in epidermis, more specifically mulG-ciliated cells. In this study, we idenGfied the transcripGonal target of RFX by performing Chip-seq analysis. We are also planning to idenGfy deferenGally regulated ciliary genes for three types of cilia formaGons. To this end, we selected several RFX genes and will discover RFX target genes involved in primary cilia, nodal cilia and mulG-cilia formtaion using Xenopus model. Key words: Ciliogenesis, ChIP-seq, Regulatory Factor X-box binding(RFX), Xenopus