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DC Field | Value | Language |
---|---|---|
dc.citation.endPage | 189 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 183 | - |
dc.citation.title | BIOCONJUGATE CHEMISTRY | - |
dc.citation.volume | 18 | - |
dc.contributor.author | Herdt, Aimee R. | - |
dc.contributor.author | Kim, Byeong-Su | - |
dc.contributor.author | Taton, T. Andrew | - |
dc.date.accessioned | 2023-12-22T09:37:36Z | - |
dc.date.available | 2023-12-22T09:37:36Z | - |
dc.date.created | 2014-11-07 | - |
dc.date.issued | 2007-01 | - |
dc.description.abstract | This paper describes the bioconjugation of histidine-tagged enzymes and other proteins to the surface of composite "magnetomicelles" consisting of magnetic γ-Fe2O3 nanoparticles encapsulated within cross-linked polystyrene-block-polyacrylate copolymer micelle shells. Free carboxylic acid groups on the magnetomicelle surface were converted to Cu2+-iminodiacetic acid (IDA) for protein capture. The conjugation of T4 DNA ligase and enhanced green fluorescent protein to magnetomicelles revealed that proteins were captured with a high surface density and could be magnetically separated from reaction mixtures and subsequently released from the nanoparticle surface. Additionally, bioconjugation of T7 RNA polymerase yielded a functional enzyme that maintained its biological activity and could be recycled for up to three subsequent transcription reactions. We propose that protein-magnetomicelle bioconjugates are effective for protein bioseparation and enzymatic recycling and further strengthen the idea that nanoparticle surfaces have utility in protein immobilization. | - |
dc.identifier.bibliographicCitation | BIOCONJUGATE CHEMISTRY, v.18, no.1, pp.183 - 189 | - |
dc.identifier.doi | 10.1021/bc060215j | - |
dc.identifier.issn | 1043-1802 | - |
dc.identifier.scopusid | 2-s2.0-33846421601 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/8463 | - |
dc.identifier.url | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=33846421601 | - |
dc.identifier.wosid | 000243503400024 | - |
dc.language | 영어 | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.title | Encapsulated magnetic nanoparticles as supports for proteins and recyclable biocatalysts | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordPlus | HISTIDINE-TAGGED PROTEINS | - |
dc.subject.keywordPlus | GREEN FLUORESCENT PROTEIN | - |
dc.subject.keywordPlus | GOLD NANOPARTICLES |
- |
dc.subject.keywordPlus | AFFINITY-CHROMATOGRAPHY | - |
dc.subject.keywordPlus | CELL-SURFACE | - |
dc.subject.keywordPlus | IMMOBILIZATION | - |
dc.subject.keywordPlus | ENZYMES | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | COORDINATION | - |
dc.subject.keywordPlus | NANOSPHERES | - |
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