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DC Field | Value | Language |
---|---|---|
dc.citation.endPage | 22 | - |
dc.citation.startPage | 13 | - |
dc.citation.title | METABOLIC ENGINEERING | - |
dc.citation.volume | 84 | - |
dc.contributor.author | Vo, Toan Minh | - |
dc.contributor.author | Park, Joon Young | - |
dc.contributor.author | Kim, Donghyuk | - |
dc.contributor.author | Park, Sunghoon | - |
dc.date.accessioned | 2024-07-16T11:05:13Z | - |
dc.date.available | 2024-07-16T11:05:13Z | - |
dc.date.created | 2024-07-08 | - |
dc.date.issued | 2024-07 | - |
dc.description.abstract | Acetate, a promising yet underutilized carbon source for biological production, was explored for the efficient production of homoserine and threonine in Escherichia coli W. A modular metabolic engineering approach revealed the crucial roles of both acetate assimilation pathways (AckA/Pta and Acs), optimized TCA cycle flux and glyoxylate shunt activity, and enhanced CoA availability, mediated by increased pantothenate kinase activity, for efficient homoserine production. The engineered strain W-H22/pM2/pR1P exhibited a high acetate assimilation rate (5.47 mmol/g cell/h) and produced 44.1 g/L homoserine in 52 h with a 53% theoretical yield (0.18 mol/mol) in fed-batch fermentation. Similarly, strain W-H31/pM2/pR1P achieved 45.8 g/L threonine in 52 h with a 65% yield (0.22 mol/mol). These results represent the highest reported levels of amino acid production using acetate, highlighting its potential as a valuable and sustainable feedstock for biomanufacturing. | - |
dc.identifier.bibliographicCitation | METABOLIC ENGINEERING, v.84, pp.13 - 22 | - |
dc.identifier.doi | 10.1016/j.ymben.2024.05.004 | - |
dc.identifier.issn | 1096-7176 | - |
dc.identifier.scopusid | 2-s2.0-85194031254 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/83180 | - |
dc.identifier.wosid | 001246502600001 | - |
dc.language | 영어 | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.title | Use of acetate as substrate for sustainable production of homoserine and threonine by Escherichia coli W3110: A modular metabolic engineering approach | - |
dc.type | Article | - |
dc.description.isOpenAccess | FALSE | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.type.docType | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | Acetate assimilation | - |
dc.subject.keywordAuthor | E. coli | - |
dc.subject.keywordAuthor | L-homoserine production | - |
dc.subject.keywordAuthor | Pathway engineering | - |
dc.subject.keywordAuthor | Two-stage fermentation | - |
dc.subject.keywordPlus | COENZYME-A | - |
dc.subject.keywordPlus | COLI | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | KINASE | - |
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