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Lee, Changsoo
Applied Biotechnology Lab for Environment
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Monitoring thiocyanate-degrading microbial community in relation to changes in process performance in mixed culture systems near washout

Author(s)
Lee, ChangsooKim, JaaiDo, HyojinHwang, Seokhwan
Issued Date
2008-02
DOI
10.1016/j.watres.2007.09.017
URI
https://scholarworks.unist.ac.kr/handle/201301/8292
Fulltext
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=38949203326
Citation
WATER RESEARCH, v.42, no.4-5, pp.1254 - 1262
Abstract
Changes in microbial community structure, associated with changes in process performance, were investigated with respect to the sludge retention time (SRT) in bioreactors treating thiocyanate. Among the seven reactors operated at 0.8-3.0 d SRTs, respectively, the reactor at 2.0 d SRT displayed the maximal thiocyanate removal rate of 240.2 mg/L/d. However, the thiocyanate removal efficiency suddenly decreased from 96.1% to 43.1% when the SRT was reduced from 2.0 to 1.8 d, corresponding to a 50.1% drop in the removal rate. Microbial communities in the reactors operated at short SRTs, near washout, were analyzed by denaturing gradient gel electrophoresis (DGGE) based on bacterial 16S rRNA genes. All band sequences recovered were assigned to two phyla, Proteobacteria and Bacteriodetes. A Thiobacillus-like microorganism was commonly detected in all the reactors and is suggested to be the main organism responsible for thiocyanate decomposition. Several DGGE band sequences were closely related to the environmental clones detected in environments rich in sulfur and/or nitrogen compounds. Statistical analysis of the DGGE profiles demonstrated that the structure of thiocyanate-degrading communities, as well as the process performance, changed with change in SRT. The microbial community profiles were not always more closely related to those at similar SRT than those at less similar SRT on the non-metric multidimensional scaling (NMDS) map. This was also supported by clustering analysis. These results were contrary to the general notion that the community structures in continuous systems will be controlled by the washout of microbial populations. Our experimental results suggest that the structure of a microbial thiocyanate-degrading community at a given SRT would not be determined only by the washout effect.
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
ISSN
0043-1354
Keyword (Author)
denaturing gradient gelelectrophoresis (DGGE)microbial community structurenon-metric multidimensionalscaling (NMDS)thiobacillus sp.thiocyanate Washout
Keyword
GRADIENT GEL-ELECTROPHORESIS16S RIBOSOMAL-RNASTRAND-CONFORMATION POLYMORPHISMOXIDIZING BACTERIAL COMMUNITIESPOLYMERASE-CHAIN-REACTIONWASTE-WATERSP. NOV.EMENDED DESCRIPTIONACTIVATED-SLUDGEDIVERSITY

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