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조윤경

Cho, Yoon-Kyoung
FRUITS Lab.
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dc.citation.endPage 2079 -
dc.citation.number 7 -
dc.citation.startPage 2069 -
dc.citation.title LAB ON A CHIP -
dc.citation.volume 24 -
dc.contributor.author Lee, Chaeeun -
dc.contributor.author Kumar, Sumit -
dc.contributor.author Park, Juhee -
dc.contributor.author Choi, Yongjun -
dc.contributor.author Clarissa, Elizabeth Maria -
dc.contributor.author Cho, Yoon-Kyoung -
dc.date.accessioned 2024-03-29T13:35:09Z -
dc.date.available 2024-03-29T13:35:09Z -
dc.date.created 2024-03-25 -
dc.date.issued 2024-04 -
dc.description.abstract The current challenge in using extracellular vesicles (EVs) as drug delivery vehicles is to precisely control their membrane permeability, specifically in the ability to switch between permeable and impermeable states without compromising their integrity and functionality. Here, we introduce a rapid, efficient, and gentle loading method for EVs based on tonicity control (TC) using a lab-on-a-disc platform. In this technique, a hypotonic solution was used for temporarily permeabilizing a membrane ("on" state), allowing the influx of molecules into EVs. The subsequent isotonic washing led to an impermeable membrane ("off" state). This loading cycle enables the loading of different cargos into EVs, such as doxorubicin hydrochloride (Dox), ssDNA, and miRNA. The TC approach was shown to be more effective than traditional methods such as sonication or extrusion, with loading yields that were 4.3-fold and 7.2-fold greater, respectively. Finally, the intracellular assessments of miRNA-497-loaded EVs and doxorubicin-loaded EVs confirmed the superior performance of TC-prepared formulations and demonstrated the impact of encapsulation heterogeneity on the therapeutic outcome, signifying potential opportunities for developing novel exosome-based therapeutic systems for clinical applications. Tonicity-induced cargo loading into extracellular vesicles (EVs) is achieved through controlled membrane permeability, ensuring cargo incorporation without causing damage to the EV membrane. -
dc.identifier.bibliographicCitation LAB ON A CHIP, v.24, no.7, pp.2069 - 2079 -
dc.identifier.doi 10.1039/d3lc00830d -
dc.identifier.issn 1473-0197 -
dc.identifier.scopusid 2-s2.0-85186967326 -
dc.identifier.uri https://scholarworks.unist.ac.kr/handle/201301/81897 -
dc.identifier.wosid 001177922300001 -
dc.language 영어 -
dc.publisher ROYAL SOC CHEMISTRY -
dc.title Tonicity-induced cargo loading into extracellular vesicles -
dc.type Article -
dc.description.isOpenAccess TRUE -
dc.relation.journalWebOfScienceCategory Biochemical Research Methods; Chemistry, Multidisciplinary; Chemistry, Analytical; Nanoscience & Nanotechnology; Instruments & Instrumentation -
dc.relation.journalResearchArea Biochemistry & Molecular Biology; Chemistry; Science & Technology - Other Topics; Instruments & Instrumentation -
dc.type.docType Article; Early Access -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -
dc.subject.keywordPlus MEMBRANE -
dc.subject.keywordPlus EXOSOMES -
dc.subject.keywordPlus CELL -
dc.subject.keywordPlus DELIVERY -
dc.subject.keywordPlus CANCER -
dc.subject.keywordPlus VEHICLES -

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