dc.citation.conferencePlace |
KO |
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dc.citation.conferencePlace |
여수 |
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dc.citation.title |
KMB 2018 45th Annual Meeting & International Symposium |
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dc.contributor.author |
Kim, Do yun |
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dc.contributor.author |
Lee, Sung Kuk |
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dc.contributor.author |
Cha, Daegeun |
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dc.date.accessioned |
2024-02-01T01:40:08Z |
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dc.date.available |
2024-02-01T01:40:08Z |
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dc.date.created |
2019-01-09 |
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dc.date.issued |
2018-06-27 |
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dc.description.abstract |
4-hydroxyvalerate (4HV) is an important compound an essential intermediate of sustainable solvents such as gamma-valerolactone (GVL) as well as a plastics additive. However, its production is limited by the high temperature reaction and the use of expensive catalysts. Thus, production of 4HV through whole-cell bioprocesses such as fermentation and biotransformation would be a promising method. Here we report the high productivity and yield of 4-hydroxyvaleric acid from the levulinic acid (LA), a cheap and renewable biomass source. For this purpose, mutant 3-hydroxybutyrate dehydrogenase (3HBDH) was expressed in Escherichia coli and 4HV production was performed from LA in glucose-fed anaerobic environment through whole cell bioconversion process. As a result, about 7 g / L of 4HV was produced, which corresponds to a 30% molar yield. In conclusion, it is expected that this study and the engineered strain will contribute to industrial plastic or gamma-valerolactone production processes. |
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dc.identifier.bibliographicCitation |
KMB 2018 45th Annual Meeting & International Symposium |
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dc.identifier.uri |
https://scholarworks.unist.ac.kr/handle/201301/81254 |
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dc.language |
영어 |
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dc.publisher |
The Korean society for Microbiology and Biotechnology |
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dc.title |
4-Hydroxyvalerate Production Using Whole-cell Biotransformation in Escherichia coli |
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dc.type |
Conference Paper |
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dc.date.conferenceDate |
2018-06-27 |
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