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Lee, Sung Kuk
Synthetic Biology & Metabolic Engineering Lab.
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dc.citation.conferencePlace KO -
dc.citation.title 2019 International Meeting of the Microbiological Society of Korea - Lee, Yong joo - Shin, Kwang Soo - Lee, Sung Kuk - 2024-02-01T00:36:42Z - 2024-02-01T00:36:42Z - 2020-01-08 - 2019-04-18 -
dc.description.abstract Microbial production of fatty-acid-derived chemicals has been studied due to its promising substitute for some petroleum-based chemicals. Many studies have attempted to engineer microbes for increased production of FFA through metabolic engineering and synthetic biology. Here, we constructed a high FFA-producing Escherichia coli (E. coli) strain by combinatorial combinations of previous positive results; 1) heterologous expression of the methylmalonyl-CoA carboxyltransferase (MMC) and overexpression of phosphoenolpyruvate carboxylase (PPC) to increase malonyl-CoA pool from oxaloacetate (OAA), 2) expressing a mutant acyl-CoA thioesterase I (‘TesA) with high specific enzyme activity, and 3) deletion of FFA transport-related genes. The manipulation of E. coli increased the FFA production by 5.4-fold compared with control strain. Additionally, employing transcriptional activator of fatty acid synthesis (FadR) further increased the TRY in our strain, resulting in 9.5- and 1.4-fold increases in FFA production, compared with the control strain expressing only the wild-type thioesterase and the positive control strain overexpressing FadR, respectively. -
dc.identifier.bibliographicCitation 2019 International Meeting of the Microbiological Society of Korea -
dc.identifier.issn 0440-2413 -
dc.identifier.uri -
dc.publisher The Microbiological Society of Korea -
dc.title Introducing Combinatorial Metabolic Engineering to Improve Free Fatty Acids Production -
dc.type Conference Paper - 2019-04-17 -


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