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Lee, Sung Kuk
Synthetic Biology & Metabolic Engineering Lab.
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Introducing Combinatorial Metabolic Engineering to Improve Free Fatty Acids Production

Lee, Yong jooShin, Kwang SooLee, Sung Kuk
Issued Date
2019 International Meeting of the Microbiological Society of Korea
Microbial production of fatty-acid-derived chemicals has been studied due to its promising substitute for some petroleum-based chemicals. Many studies have attempted to engineer microbes for increased production of FFA through metabolic engineering and synthetic biology. Here, we constructed a high FFA-producing Escherichia coli (E. coli) strain by combinatorial combinations of previous positive results; 1) heterologous expression of the methylmalonyl-CoA carboxyltransferase (MMC) and overexpression of phosphoenolpyruvate carboxylase (PPC) to increase malonyl-CoA pool from oxaloacetate (OAA), 2) expressing a mutant acyl-CoA thioesterase I (‘TesA) with high specific enzyme activity, and 3) deletion of FFA transport-related genes. The manipulation of E. coli increased the FFA production by 5.4-fold compared with control strain. Additionally, employing transcriptional activator of fatty acid synthesis (FadR) further increased the TRY in our strain, resulting in 9.5- and 1.4-fold increases in FFA production, compared with the control strain expressing only the wild-type thioesterase and the positive control strain overexpressing FadR, respectively.
The Microbiological Society of Korea


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