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Cha, Chaenyung
Integrative Biomaterials Engineering
Research Interests
  • Biopolymer, nanocomposites, microfabrication, tissue engineering, drug delivery

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Top-down Synthesis of Versatile Polyaspartamide Linkers for Single-Step Protein Conjugation to Materials

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dc.contributor.author Cha, Chaenyung ko
dc.contributor.author Jeong, Jae Hyun ko
dc.contributor.author Tang, Xin ko
dc.contributor.author Zill, Andrew T. ko
dc.contributor.author Prakash, Y. S. ko
dc.contributor.author Zimmerman, Steven C. ko
dc.contributor.author Saif, Taher A. ko
dc.contributor.author Kong, Hyunjoon ko
dc.date.available 2014-10-29T00:20:20Z -
dc.date.created 2014-10-27 ko
dc.date.issued 2011-12 -
dc.identifier.citation BIOCONJUGATE CHEMISTRY, v.22, no.12, pp.2377 - 2382 ko
dc.identifier.issn 1043-1802 ko
dc.identifier.uri https://scholarworks.unist.ac.kr/handle/201301/7875 -
dc.identifier.uri http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84555194829 ko
dc.description.abstract Materials used in various biological applications are often modified with proteins to regulate biomolecular and cellular adhesion. Conventional strategies of protein conjugation accompany monovalent bifunctional protein linkers, which present several limitations in molecular synthesis and protein conjugation. Herein, we present a new strategy of preparing multivalent polyaspartamide linkers in a simple top-down manner, and also demonstrate that the resulting polymer linkers allow us to readily conjugate proteins to both organic and inorganic materials. The top-down synthesis of polyaspartamide linkers was performed by partially opening succinimidyl ring moieties of polysuccinimide (PSI) with the controlled number of nucleophiles reactive to photo-cross-linked hydrogel or gold-coated inorganic materials: (1) Poly(2-hydroxyethyl-co-2-methacryloxyethyl aspartamide) (PHMAA) presenting methacrylate was used to micropattern fibronectin or collagen on a hydrogel in order to regulate cell adhesion and growth area on a micrometer scale. (2) Poly(2-hydroxyethyl-co-2-mercaptoethyl aspartamide) (PHMCA) presenting thiol functional groups was used to link fibronectin to a gold-coated silicon microelectromechanical probe designed to measure cell traction force. Overall, these multivalent polyaspartamide protein linkers will greatly assist efforts to analyze and regulate the cellular adhesion to and phenotypic activities of a wide array of substrates and devices. ko
dc.description.statementofresponsibility close -
dc.language ENG ko
dc.publisher AMER CHEMICAL SOC ko
dc.subject FOCAL ADHESIONS ko
dc.subject HYDROGELS ko
dc.subject BIOMATERIALS ko
dc.subject DESIGN ko
dc.subject MONOLAYERS ko
dc.subject BEHAVIOR ko
dc.subject ACID ko
dc.title Top-down Synthesis of Versatile Polyaspartamide Linkers for Single-Step Protein Conjugation to Materials ko
dc.type ARTICLE ko
dc.identifier.scopusid 2-s2.0-84555194829 ko
dc.identifier.wosid 000298222300003 ko
dc.type.rims ART ko
dc.description.wostc 8 *
dc.description.scopustc 9 *
dc.date.tcdate 2015-05-06 *
dc.date.scptcdate 2014-10-27 *
dc.identifier.doi 10.1021/bc200339s ko
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