Quantitative Analysis of Mouse Organs Using Coherence Gating Optical Imaging and Tissue Clearing Technique

Abstract

Optical imaging techniques with physical tissue sectioning have become indispensable tools. However, acquiring volumetric anatomy of multiple organs and statistical studies remain a difficult challenge due to light scattering and long data acquisition period. Here, we propose a novel protocol for the high-throughput and quantitative analysis of 3D mouse organs using Coherence gating imaging (CGI) with tissue clearing. For statistical analysis, we also applied deep learning and outcomes were compared with computed tomography. Our preliminary results can improve imaging depth as well corresponding acquisition time, which would be promising tool for 3D digital histopathology.