Production of D-Lactate Utilizing Multiple Sugars Derived from Biomass by Engineered E. coli

Abstract

Co-utilizing of multiple carbon sources have been interested for decades in the synthetic biology field. To suppress the greenhouse effect, people have tried to find renewable energy sources. Biomass is the representative alternative carbon source, but pre-treatment and reprocessing steps of biomass are necessary to convert cellulose, and hemicellulose to sugars. Cellulose of biomass can be converted to glucose, and hemicellulose of biomass can be converted to xylose and arabinose. Constructing strain which can co-utilize multiple sugars is not only reducing pre-treatment cost but also increasing productivity of bio-chemical. We constructed two type of strains. One is strain which can co-utilize glucose, xylose and cellobiose by transformation of cellobiose pathway from Enterobacter Cloacae, and the other is strain which can co-utilize glucose, xylose and arabinose by genome engineering on araB promoter region. In this study, the research was conducted to confirm the ability to co-utilize multiple sugars and to produce lactic acid.