BROWSE

Related Researcher

Author's Photo

Park, Tae Joo
Developmental Morphogenesis Lab
Research Interests
  • Morphogenesis, chondrogenesis, ciliogenesis

ITEM VIEW & DOWNLOAD

Identification of novel ciliogenesis factors using a new in vivo model for mucociliary epithelial development

Cited 49 times inthomson ciCited 0 times inthomson ci
Title
Identification of novel ciliogenesis factors using a new in vivo model for mucociliary epithelial development
Author
Hayes, Julie M.Kim, Su KyoungAbitua, Philip B.Park, Tae JooHerrington, Emily R.Kitayama, AtsushiGrow, Matthew W.Ueno, NaotoWallingford, John B.
Issue Date
2007-12
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Citation
DEVELOPMENTAL BIOLOGY, v.312, no.1, pp.115 - 130
Abstract
Mucociliary epithelia are essential for homeostasis of many organs and consist of mucus-secreting goblet cells and ciliated cells. Here, we present the ciliated epidermis of Xenopus embryos as a facile model system for in vivo molecular studies of mucociliary epithelial development. Using an in situ hybridization-based approach, we identified numerous genes expressed differentially in mucus-secreting cells or in ciliated cells. Focusing on genes expressed in ciliated cells, we have identified new candidate ciliogenesis factors, including several not present in the current ciliome. We find that TTC25-GFP is localized to the base of cilia and to ciliary axonemes, and disruption of TTC25 function disrupts ciliogenesis. Mig12-GFP localizes very strongly to the base of cilia and confocal imaging of this construct allows for simple visualization of the planar polarity of basal bodies that underlies polarized ciliary beating. Knockdown of Mig12 disrupts ciliogenesis. Finally, we show that ciliogenesis factors identified in the Xenopus epidermis are required in the midline to facilitate neural tube closure. These results provide further evidence of a requirement for cilia in neural tube morphogenesis and suggest that genes identified in the Xenopus epidermis play broad roles in ciliogenesis. The suites of genes identified here will provide a foundation for future studies, and may also contribute to our understanding of pathological changes in mucociliary epithelia that accompany diseases such as asthma.
URI
https://scholarworks.unist.ac.kr/handle/201301/7179
URL
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=36549028333
DOI
10.1016/j.ydbio.2007.09.031
ISSN
0012-1606
Appears in Collections:
BIO_Journal Papers
Files in This Item:
There are no files associated with this item.

find_unist can give you direct access to the published full text of this article. (UNISTARs only)

Show full item record

qrcode

  • mendeley

    citeulike

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

MENU